Guanidine Hydrochloride

Guanidine Hydrochloride

Synonyms Aminoformamidine hydrochloride; Aminomethanamidine hydrochloride; Guanidinium chloride; Guanidium chloride; Guanidine HCl

CAS Number: 50-01-1 Molecular Formula: CH5N3·HCl Molecular Weight: 95.53 g/mol

Beilstein Registry Number: 3591990 EC Number: 200-002-3 MDL Number: MFCD00013026

Configure Your Product:

SKU 02151210-CF
Price:
$22.75
In Stock
Request a quote
Description

Product Description

Guanidine Hydrochloride

Application Notes

Guanidine Hydrochloride is a strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies and be used for the recovery of periplasmic proteins. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary. It is also used in the isolation of RNA to dissociate the nucleoprotein into its nucleic acid and protein moieties. It is an inhibitor of RNase.

Usage Statement

Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.

Key Applications

Strong denaturant

Specifications
SKU 02151210-CF
Application Notes Guanidine Hydrochloride is a strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies and be used for the recovery of periplasmic proteins. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary. It is also used in the isolation of RNA to dissociate the nucleoprotein into its nucleic acid and protein moieties. It is an inhibitor of RNase.
Beilstein Registry Number 3591990
Density 1.3 g/cm³
EC Number 200-002-3
Format Crystalline powder
Grade Optical Density Grade
Hazard Statements H302-H315-H319
Applications Strong denaturant
Melting Point 178-185°C
Molecular Formula CH5N3·HCl
Molecular Weight 95.53 g/mol
Personal Protective Equipment Dust mask, Eyeshields, Faceshields, Gloves
Physical Appearance White Crystalline Powder
pKa 13.6 (Lit.)
Product Families Description Guanidine hydrochloride
Product Overview Guanidine Hydrochloride
Purity ~99%
RTECS Number MF4300000
Safety Symbol GHS07
Solubility in <a class="pubchem-internal-link CID-962" href="https://pubchem.ncbi.nlm.nih.gov/compound/water">water</a>, g/100ml at 20°C: 215
Storage and Handling Store at Room Temperature (15-30°C). Store Desiccated.
Typical Working Concentration Highly concentrated (6 - 8 M) Guanidine HCl solutions are used to denature native globular proteins.
Usage Statement Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.
UV Visible Absorbance OD260 (6.0 M aq soln): ≤0.03