Dino

Concanavalin A

Concanavalin A

Synonyms Con A

CAS Number: 11028-71-0 Molecular Weight: 6312.315 g/mol

EC Number: 234-258-2 MDL Number: MFCD00071069

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SKU 02150710-CF
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Description

Product Description

Concanavalin A

Application Notes

Lectin are used in a wide variety of applications in vitro, including: blood grouping and erythrocyte polyagglutination studies, Mitogenic stimulation of lymphocytes, Lymphocyte subpopulation studies, Fractionation of cells and other particles, Histochemical studies of normal and pathological conditions. Con-A has been used to elucidate structural changes in the membrane surface of transformed cells. Con-A conjugates of antitumor drugs have been used in drug delivery systems in cultured cells.

Usage Statement

Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.

Key Applications

Cell Biology||Cell Signaling

Specifications
SKU 02150710-CF
Application Notes Lectin are used in a wide variety of applications in vitro, including: blood grouping and erythrocyte polyagglutination studies, Mitogenic stimulation of lymphocytes, Lymphocyte subpopulation studies, Fractionation of cells and other particles, Histochemical studies of normal and pathological conditions. Con-A has been used to elucidate structural changes in the membrane surface of transformed cells. Con-A conjugates of antitumor drugs have been used in drug delivery systems in cultured cells.
Biochemical Physiological Actions Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6. Con-A binds specifically to mannosyl and glucosyl residues of polysaccharides and glycoproteins. Unmodified hydroxyl groups at the C3, C4 and C6 positions of D-glucopyranosyl or D-mannopyranosyl rings may be essential for binding. Each subunit of Con-A contains one calcium ion and one manganese ion. Removal of these cations by dialysis under acidic conditions abolishes the carbohydrate-binding activity.
EC Number 234-258-2
Extinction Coefficient 280nm = 11.4 (0.1 M NaCl)(Lit.)
Format powder
Hazard Statements H317-H334-H361
Isoelectric Point 4.5, 4.7, 5.0-5.1, and 5.4-5.5 (several isoforms)(Lit.)
Applications Cell Biology||Cell Signaling
Molecular Weight 6312.315 g/mol
Personal Protective Equipment Dust mask, Eyeshields, Faceshields, Gloves
Physical Appearance White Shiny Powder
Product Families Description Concanavalin A
Product Overview Concanavalin A
RTECS Number GK6890000
Safety Symbol GHS08
Source Canavalia ensiformis seeds (jack beans)
Specificity Carbohydrate Specificity: a-Mannose; a-Glucose; Branched Mannose.
Storage and Handling -20 °C
Typical Working Concentration Reconstitution: Reconstitute with buffer to a final concentration of 1 mg/mL.
Usage Statement Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.