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Collagenase Type 3

Collagenase Type 3

Synonyms Clostridiopeptidase A; EC 3.4.24.3

CAS Number: 2593923

EC Number: 232-582-9 MDL Number: MFCD00130830

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SKU 02150704-CF
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Description

Product Description

Collagenase Type 3

Application Notes

Collagenase has found widespread application in the isolation of specific cell types from attendant connective tissue. Most preparations contain collagenase, non-specifc protease, and clostripain activities. These enzymes act synergistically to degrade collagen and other intercellular material. Crude Collagenases are suitable for isolation of cells from many types of animal tissue. The wide spectrum of activity of crude collagenase makes it especially useful as a debriding agent.

Usage Statement

Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.

Key Applications

Cell Dissociation||Cell Lysis

Specifications
SKU 02150704-CF
Application Notes Collagenase has found widespread application in the isolation of specific cell types from attendant connective tissue. Most preparations contain collagenase, non-specifc protease, and clostripain activities. These enzymes act synergistically to degrade collagen and other intercellular material. Crude Collagenases are suitable for isolation of cells from many types of animal tissue. The wide spectrum of activity of crude collagenase makes it especially useful as a debriding agent.
Biochemical Physiological Actions Collagenase is activated by four gram atom calcium per mole enzyme. It is inhibited by ethylene glycol-bis(beta-aminoethyl ether) - N, N, N',N'-tetraacetic acid, beta-mercaptoethanol, glutathione, thioglycolic acid and 8-hydroxyquinoline. Crude collagenases are mixtures of enzymes (mostly proteases) secreted by C. histolyticum. Effective release of cells from tissue requires the action of both collagenase enzymes and the neutral protease. Collagenase is activated by four gram atom calcium (Ca2+) per mole enzyme. The culture filtrate is thought to contain at least 7 different proteases ranging in molecular weight from 68-130 kDa.
EC Number 232-582-9
Format powder
Hazard Statements H315-H319-H334-H335
Applications Cell Dissociation||Cell Lysis
Personal Protective Equipment Dust mask, Eyeshields, Faceshields, Gloves
Physical Appearance Brown Lyophilized Powder
Product Families Description Collagenase
Product Overview Collagenase Type 3
Safety Symbol GHS07, GHS08
Source Clostridium histolyticum
Specific Activity >100 u/mg
Storage and Handling Store at +4 °C; desiccate.
Typical Working Concentration For typical assay: Dissolve enzyme at a concentration of 1 mg/mL in 0.05 M TES with 0.36 mM calcium chloride, pH 7.5. Dilutions run are 1/10 and 1/20 in the above buffer.
Unit Definition One unit liberates one µmole of L-leucine equivalents from collagen in 5 hours at 37 °C, pH 7.5.
Usage Statement Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.