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Cholesterol Oxidase

Cholesterol Oxidase

Synonyms E.C. 1.1.3.6; Cholesterol : oxygen oxidoreductase; 3b-hydroxy steroid oxidoreductase; CHOD; 3b-hydroxysteroid:oxygen oxidoreductase; cholesterol-O2 oxidoreductase

CAS Number: 9028-76-6

EC Number: 232-842-1 MDL Number: MFCD00130783

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SKU 02150674-CF
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Description

Product Description

Cholesterol Oxidase

Application Notes

Cholesterol oxidase from Streptomyces has been used in a study to assess the relationship between the micellar structure of model bile and the activity of esterase. Cholesterol oxidase from Streptomyces has also been used in a study to investigate the effects of sphingomyelin degradation on cell cholesterol oxidizability and steady-state distribution between the cell surface and the cell interior. This enzyme is useful for the determination of cholesterol in serum by coupling with cholesterol esterase. CHOD is used to determine serum cholesterol. It is the second most widely used enzyme in clinical applications after glucose oxidase. CHOD also finds application in the microanalysis of steroids in food samples and in distinguishing 3-ketosteroids from 3b-hydroxysteroids. Transgenic plants expressing cholesterol oxidase are being investigated in the fight against the cotton boll weevil. Cholesterol oxidase has also been used as a molecular probe to elucidate cellular membrane structures.

Usage Statement

Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.

Key Applications

Enzymes||Inhibitors||Substrates

Specifications
SKU 02150674-CF
Application Notes Cholesterol oxidase from Streptomyces has been used in a study to assess the relationship between the micellar structure of model bile and the activity of esterase. Cholesterol oxidase from Streptomyces has also been used in a study to investigate the effects of sphingomyelin degradation on cell cholesterol oxidizability and steady-state distribution between the cell surface and the cell interior. This enzyme is useful for the determination of cholesterol in serum by coupling with cholesterol esterase. CHOD is used to determine serum cholesterol. It is the second most widely used enzyme in clinical applications after glucose oxidase. CHOD also finds application in the microanalysis of steroids in food samples and in distinguishing 3-ketosteroids from 3b-hydroxysteroids. Transgenic plants expressing cholesterol oxidase are being investigated in the fight against the cotton boll weevil. Cholesterol oxidase has also been used as a molecular probe to elucidate cellular membrane structures.
Biochemical Physiological Actions Cholesterol oxidase (CHOD) is a monomeric flavoprotein containing FAD that catalyzes the first step in cholesterol catabolism. This bifunctional enzyme oxidizes cholesterol to cholest-5-en-3-one in an FAD-requiring step, which is then isomerized to cholest-4-en-3-one with the release of H2O2.
EC Number 232-842-1
Format powder
Isoelectric Point 5.1 ± 0.1 and 5.4 ± 0.1(Lit.)
Applications Enzymes||Inhibitors||Substrates
Personal Protective Equipment Eyeshields, Gloves, Respirator filter
Physical Appearance Dark Yellow Powder
Product Families Description Cholesterol Oxidase
Product Overview Cholesterol Oxidase
Source Steptomyces sp.
Specific Activity ~15 u/mg solid
Storage and Handling 0°C
Unit Definition One unit causes the formation of one µmole of hydrogen peroxide per minute at pH 7.0 and 37 °C.
Usage Statement Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.