Poly-D-Lysine Hydrobromide

Poly-D-Lysine Hydrobromide

CAS Number: 27964-99-4

MDL Number: MFCD00131934

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SKU 02150175-CF
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Description

Product Description

Poly-D-Lysine Hydrobromide

Application Notes

It is typically used as a coating substrate for culture dishes, slides, etc. It enhances electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. Both the D- and L- form of the poly-lysine can be used as a coating substrate since poly-lysine is a nonspecific attachment factor for cells; however, certain cells can digest poly-lysine. In this case, poly-D-lysine should be used as the attachment factor so that the cells are not disrupted by excessive uptake of L-lysine.Other uses for poly-lysine have been reported as well:• Conjugation to methotrexate for increased drug transport.• Microencapsulation of islets.• Use in simple reproducible procedure for chromosomal preparations from a variety of tissues.• Immobilation of living cells in biocompatible semipermeable microcapsules.• In the preparation of polycationic beads.• Conjugation to albumin and horseradish peroxidase to enhance cellular uptake.

Usage Statement

Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.

Key Applications

Cell adhesion

Specifications
SKU 02150175-CF
Analysis Note Molecular weight based on viscosity. Also assayed by MALLS.
Application Notes It is typically used as a coating substrate for culture dishes, slides, etc. It enhances electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. Both the D- and L- form of the poly-lysine can be used as a coating substrate since poly-lysine is a nonspecific attachment factor for cells; however, certain cells can digest poly-lysine. In this case, poly-D-lysine should be used as the attachment factor so that the cells are not disrupted by excessive uptake of L-lysine.Other uses for poly-lysine have been reported as well:• Conjugation to methotrexate for increased drug transport.• Microencapsulation of islets.• Use in simple reproducible procedure for chromosomal preparations from a variety of tissues.• Immobilation of living cells in biocompatible semipermeable microcapsules.• In the preparation of polycationic beads.• Conjugation to albumin and horseradish peroxidase to enhance cellular uptake.
Biochemical Physiological Actions Poly-D-lysine hydrobromide is a synthetic amino acid that enhances cell adhesion to solid substrates. It has additionally been shown to eliminate prion proteins from infected cells.
Applications Cell adhesion
MDL # MFCD00131934
Personal Protective Equipment Eyeshields, Gloves,respirator filter
Physical Appearance White Powder
Product Families Description Poly-D-lysine hydrobromide
Product Overview Poly-D-Lysine Hydrobromide
Storage and Handling -20°C
Usage Statement Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.