Dino

5-Bromo-4-Chloro-3-Indolyl Phosphate Disodium Salt

5-Bromo-4-Chloro-3-Indolyl Phosphate Disodium Salt

Synonyms BCIP; X-phosphate disodium salt

CAS Number: 102185-33-1 Molecular Formula: C8H4BrClNO4PNa2 Molecular Weight: 370.43 g/mol

MDL Number: MFCD00036757

Configure Your Product:

SKU 02150042-CF
Price:
$48.70
In Stock
Request a quote
Description

Product Description

5-Bromo-4-Chloro-3-Indolyl Phosphate Disodium Salt

Application Notes

For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry. BCIP disodium salt is soluble in water. It may be used to prepare a stock solution, which in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color.

Usage Statement

Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.

Key Applications

Substrate for alkaline phosphatase

Specifications
SKU 02150042-CF
Application Notes For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry. BCIP disodium salt is soluble in water. It may be used to prepare a stock solution, which in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color.
Format powder
Applications Substrate for alkaline phosphatase
Loss on Drying ≤10%
Molecular Formula C8H4BrClNO4PNa2
Molecular Weight 370.43 g/mol
Personal Protective Equipment Eyeshields, Gloves, respirator filter
Physical Appearance Off-white Powder
Product Families Description 5-Bromo-4-chloro-3-indolyl phosphate disodium salt
Product Overview 5-Bromo-4-Chloro-3-Indolyl Phosphate Disodium Salt
Purity ≥99%
Storage and Handling -20°C; desiccate; protect from light
Usage Statement Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.
UV Visible Absorbance λ max (water): 291 ± 3 nm