Cholesterol Esterase

Cholesterol Esterase

Synonyms Bile salt activated lipase; sterol esterase; carboxyl ester lipase; steryl-ester acylhydrolase; Cholesterase; Neutral cholesteryl ester hydrolase

CAS Number: 9026-00-0

EC Number: 232-808-6 MDL Number: MFCD00071052

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SKU 02105439-CF
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Description

Product Description

Cholesterol Esterase

Application Notes

Cholesterol Esterase is useful for enzymatic determination of total cholesterol when coupled with cholesterol oxidase in clinical analysis.

Usage Statement

Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.

Key Applications

Synthesis of fatty acid esters of cholesterol

Specifications
SKU 02105439-CF
Application Notes Cholesterol Esterase is useful for enzymatic determination of total cholesterol when coupled with cholesterol oxidase in clinical analysis.
Biochemical Physiological Actions Cholesterol esterase (CE) is a reversible enzyme that can hydrolyze or synthesize fatty acid esters of cholesterol and other sterols. Hydrolysis of water insoluble long chain fatty acid esters requires bile salt activation. Hydrolysis of water soluble esters of short chain fatty acids and lysophospholipids does not require activation by bile salts. It also hydrolyzes tri-, di-, and mono-acylglycerols, phospholipids, lysophospholipids, and ceramide. The enzyme may have multiple functions in lipid and lipoprotein metabolism, and atherosclerosis.
EC Number 232-808-6
Format lyophilized powder
Isoelectric Point 5.9 ± 0.1(Lit.)
Applications Synthesis of fatty acid esters of cholesterol
Personal Protective Equipment Eyeshields, Gloves, Respirator filter.
Physical Appearance Yellowish Tan Lyophilized Powder
Product Families Description Cholesterol Esterase
Product Overview Cholesterol Esterase
Protein or Enzyme Type Proteins, Enzymes & Peptides
Source Pseudomonas species
Specific Activity ≥100 u/mg solid
Storage and Handling 0 °C
Unit Definition One unit is the amount of enzyme that will hydrolyze 1.0 micromole of cholesterol ester per minute at 37 °C, pH 7.0.
Usage Statement Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.