Cholesterol Esterase is useful for enzymatic determination of total cholesterol when coupled with cholesterol oxidase in clinical analysis.
Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.
Synthesis of fatty acid esters of cholesterol
|Application Notes||Cholesterol Esterase is useful for enzymatic determination of total cholesterol when coupled with cholesterol oxidase in clinical analysis.|
|Biochemical Physiological Actions||Cholesterol esterase (CE) is a reversible enzyme that can hydrolyze or synthesize fatty acid esters of cholesterol and other sterols. Hydrolysis of water insoluble long chain fatty acid esters requires bile salt activation. Hydrolysis of water soluble esters of short chain fatty acids and lysophospholipids does not require activation by bile salts. It also hydrolyzes tri-, di-, and mono-acylglycerols, phospholipids, lysophospholipids, and ceramide. The enzyme may have multiple functions in lipid and lipoprotein metabolism, and atherosclerosis.|
|Isoelectric Point||5.9 ± 0.1(Lit.)|
|Applications||Synthesis of fatty acid esters of cholesterol|
|Personal Protective Equipment||Eyeshields, Gloves, Respirator filter.|
|Physical Appearance||Yellowish Tan Lyophilized Powder|
|Product Families Description||Cholesterol Esterase|
|Product Overview||Cholesterol Esterase|
|Protein or Enzyme Type||Proteins, Enzymes & Peptides|
|Specific Activity||≥100 u/mg solid|
|Storage and Handling||0 °C|
|Unit Definition||One unit is the amount of enzyme that will hydrolyze 1.0 micromole of cholesterol ester per minute at 37 °C, pH 7.0.|
|Usage Statement||Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.|