Albumin, Bovine

Albumin, Bovine

Synonyms BSA; Bovine albumin; Bovine Plasma Albumin

CAS Number: 9048-46-8

EC Number: 232-936-2 MDL Number: MFCD00130384

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SKU 02105033-CF
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Description

Product Description

Albumin, Bovine

Application Notes

A fatty acid free bovine albumin powder designed for the most sensitive research and diagnostic applications. Manufactured by a process specifically designed to give consistently low levels of residual metabolites and enzymes. Especially suitable for RIA and enzyme kits as a protein base. Albumin is used to solubilize lipids, stabilize protein solutions, and is also used as a blocking agent in Western blots or ELISA applications. Globulin free albumins are suitable for use in applications where no other proteins should be present such as electrophoresis. Insulin RIA: 1 micro unit/gm T3 and T4 at or below detectable levels.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals

Key Applications

Antibody Purification||Blood banking reagents||Electrophoresis standard||ELISA||Protein Standard||Protein base or filler

Specifications
SKU 02105033-CF
Alternate Names BSA; Bovine albumin; Bovine Plasma Albumin
Application Notes A fatty acid free bovine albumin powder designed for the most sensitive research and diagnostic applications. Manufactured by a process specifically designed to give consistently low levels of residual metabolites and enzymes. Especially suitable for RIA and enzyme kits as a protein base. Albumin is used to solubilize lipids, stabilize protein solutions, and is also used as a blocking agent in Western blots or ELISA applications. Globulin free albumins are suitable for use in applications where no other proteins should be present such as electrophoresis. Insulin RIA: 1 micro unit/gm T3 and T4 at or below detectable levels.
Biochemical Physiological Actions Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies. Albumin binds water, Ca2+, Na+ and K+. Due to a hydrophobic cleft, albumin binds fatty acids, bilirubin, hormones and drugs. The main biological function of albumin is to regulate the colloidal osmotic pressure of blood and to a lesser degree to provide cellular nutrition. Human and bovine albumins contain 16% nitrogen and are often used as standards in protein calibration studies.
EC Number 232-936-2
Grade RIA Grade
Isoelectric Point (Water at 25 °C) Endogenous material - 4.7, 4.9; Fatty acid depleted - 5.3
Applications Antibody Purification||Blood banking reagents||Electrophoresis standard||ELISA||Protein Standard||Protein base or filler
MDL # MFCD00130384
Moisture Content ≤5%
Optical Rotation ≤0.24 mg/g
Physical Appearance Off-white to Light Green Crystalline Powder
pKa 6.8 - 7.2 (1% sln/0.15M NaCl)
Product Families Description Albumin
Product Overview Albumin, Bovine
Purity ≥98%; Protein ≥96%
RTECS Number MT6446000
Source Albumins are readily soluble in water and can only be precipitated by high concentrations of neutral salts such as ammonium sulfate.
Specific Activity Bovine Serum
Storage and Handling +4 °C
Sulphated Ash Suitable for insulin RIA procedures
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals