Dino

Saponin

Saponin

Synonyms Sapogenin Glycosides

CAS Number: 8047-15-2

EC Number: 232-462-6 MDL Number: MFCD00081981

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SKU 02102855-CF
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Description

Product Description

Saponin

Application Notes

1% of saponin solution can hemolyze erythrocytes. Saponin solutions have been used in the following ways: concentrations of 0.005%-0.01% were used to permeabilize cultured human intestinal epithelial cells after incubation for 30' prior to immunofluorescence; a 0.05% solution of saponin was reportedly used to permeabilize paraformaldehyde-saponin- fixed human fibroblasts for the staining of intracellular fibronectin by different types of peroxidase conjugates for light and electron microscopy; saponins, a surface-active agent, have also been used to lyse the outer membranes of Rous sarcoma viruses, cell membranes of chicken liver and erythrocytes of human and guinea pig. It also has been used as an adjuvant for vaccines.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Hemolyze erythrocytes||Permeabilize cultured human intestinal epithelial cells and paraformaldehyde-saponin- fixed human fibroblasts||Surface-active agent

Specifications
SKU 02102855-CF
Application Notes 1% of saponin solution can hemolyze erythrocytes. Saponin solutions have been used in the following ways: concentrations of 0.005%-0.01% were used to permeabilize cultured human intestinal epithelial cells after incubation for 30' prior to immunofluorescence; a 0.05% solution of saponin was reportedly used to permeabilize paraformaldehyde-saponin- fixed human fibroblasts for the staining of intracellular fibronectin by different types of peroxidase conjugates for light and electron microscopy; saponins, a surface-active agent, have also been used to lyse the outer membranes of Rous sarcoma viruses, cell membranes of chicken liver and erythrocytes of human and guinea pig. It also has been used as an adjuvant for vaccines.
Biochemical Physiological Actions Potent hemolytic when injected i.v.; surfactant that enhances penetration of proteins and other macromolecules through cell membranes
EC Number 232-462-6
Format powder
Grade Practical Grade
Hazard Statements H319-H335
Applications Hemolyze erythrocytes||Permeabilize cultured human intestinal epithelial cells and paraformaldehyde-saponin- fixed human fibroblasts||Surface-active agent
Loss on Drying ≤10%
Personal Protective Equipment Dust mask, Eyeshields, Gloves
Physical Appearance White Powder
Product Families Description Saponin
Product Overview Saponin
RTECS Number VQ1400000
Safety Symbol GHS07
Source Plant
Storage and Handling Store at Room Temperature (15-30 °C).
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.