Pepsin

Pepsin

Synonyms Pepsin A

CAS Number: 9001-75-6

EC Number: 232-629-3 MDL Number: pH 1.0

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SKU 02102598-CF
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Description

Product Description

Pepsin

Application Notes

Pepsin is a peptidase used to digest proteins and is commonly used in the preparation of Fab fragments from antibodies. Pepsin, from porcine gastric mucosa, has been used to hydrolyze dry cervical samples in mice.

Usage Statement

Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.

Key Applications

Pepsin is used as a proteolytic enzyme; used to break down the proteins into their components; i.e.; peptides and aminoacids

Specifications
SKU 02102598-CF
Alternate Names Pepsin A
Application Notes Pepsin is a peptidase used to digest proteins and is commonly used in the preparation of Fab fragments from antibodies. Pepsin, from porcine gastric mucosa, has been used to hydrolyze dry cervical samples in mice.
Biochemical Physiological Actions Pepsin hydrolyzes peptide bonds, not amide or ester linkages. Pepsin cleaves peptides with an aromatic acid on either side of the peptide bond. Sulfur-containing amino acids increase susceptibility to hydrolysis when they are close to the peptide bond. Pepsin preferentially cleaves at the carboxyl side of phenylalanine and leucine and at the carboxyl side of glutamic acid residues. Cleaves Phe-Val, Gln-His, Glu-Ala, Ala-Leu, Leu-Tyr, Tyr-Leu, Gly-Phe, Phe-Phe and Phe-Tyr bonds in the β chain of insulin.Pepsin is the major proteolytic enzyme produced in the stomach. It digests proteins through the cleavage of interior peptide linkages.
EC Number 232-629-3
Extinction Coefficient E1%280 = 14.7
Foreign Activity E. coli
Format NegativeSalmonella
Gel Strength Negative
Gel Temperature Powder
Insoluble Matter H315-H319-H334-H335
Applications Pepsin is used as a proteolytic enzyme; used to break down the proteins into their components; i.e.; peptides and aminoacids
MDL # pH 1.0
Moisture Content MFCD00081840
Optical Rotation 0210259850||0210259880||0210259890||0210259891
Physical Appearance Off-white Lyophilized Powder
pKa dust mask, Eyeshields, Faceshields, Gloves
Potency 2.5-4.5 (2% aq soln)
Product Families Description Pepsin
Product Overview Pepsin
Protein or Enzyme Type Proteins, Enzymes & Peptides
RTECS Number SC6132000
Source Danger
Specific Activity Freely soluble in water; dissolves readily in 0.01 M HCl (0.5 mg/mL - clear, colorless solution); practically insoluble in alcohol, chloroform or ether.
Sterility Porcine
Sterilization of Solutions ≥1:10,000
Storage and Handling Room Temperature
Usage Statement Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.