Dino

Papain

Papain

Synonyms Papayotin; Vegetable Pepsin; Caroid; Vermizym; Papainase

CAS Number: 9001-73-4 Molecular Weight: 226.236 g/mol

EC Number: 232-627-2 MDL Number: MFCD00131791

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SKU 02102566-CF
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Description

Product Description

Papain

Application Notes

Papain is widely used in cell isolation. With some tissues papain has proved less damaging and more effective than other proteases. Of the enzymes used for dissociating turtle retina, papain produced the least trauma. Intact single photoreceptor cells have also been isolated from adult salamander retina with papain. Papain used to obtain high yields of viable, morphologically intact cortical neurons from postnatal rats. Used for dissociation of postnatal rat hippocampus. Papain is used with fetal as well as postnatal brain regions to provide maximal dissociation and viability of neurons. It is also used to produce Fab fragments of antibodies. In addition, papain is used in red cell serology to modify the red cell surface to enhance or destroy the reactivity of many red cell antigens. Papain is useful for platelet serology studies. Papain has also been used in the enzymatic synthesis of amino acids, peptides, and other molecules.

Usage Statement

Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.

Key Applications

For production of Fab fragments of antibodies||Used for cell dissociation

Specifications
SKU 02102566-CF
Application Notes Papain is widely used in cell isolation. With some tissues papain has proved less damaging and more effective than other proteases. Of the enzymes used for dissociating turtle retina, papain produced the least trauma. Intact single photoreceptor cells have also been isolated from adult salamander retina with papain. Papain used to obtain high yields of viable, morphologically intact cortical neurons from postnatal rats. Used for dissociation of postnatal rat hippocampus. Papain is used with fetal as well as postnatal brain regions to provide maximal dissociation and viability of neurons. It is also used to produce Fab fragments of antibodies. In addition, papain is used in red cell serology to modify the red cell surface to enhance or destroy the reactivity of many red cell antigens. Papain is useful for platelet serology studies. Papain has also been used in the enzymatic synthesis of amino acids, peptides, and other molecules.
Biochemical Physiological Actions Papain is a cysteine protease that cleaves peptide bonds of basic amino acids, leucine, or glycine. It is also hydrolyzes esters and amides. Papain consists of a single polypeptide chain with three disulfide bridges and a sulfhydryl group necessary for activity of the enzyme.
EC Number 232-627-2
Extinction Coefficient 25.0 (Lit.)
Format powder
Grade Technical
Hazard Statements H315-H319-H334-H335
Isoelectric Point pH 9.6 (Lit.)
Applications For production of Fab fragments of antibodies||Used for cell dissociation
Loss on Drying ≤8%
Molecular Weight 226.236 g/mol
Personal Protective Equipment Dust mask, Eyeshields, Faceshields, Gloves
Physical Appearance Powder
Product Families Description Papain
Product Overview Papain
RTECS Number RU4950000
Safety Symbol GHS07, GHS08
Specific Activity >1750 USP u/mg
Storage and Handling Store at +4 °C; desiccate.
Usage Statement Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.