O-Nitrophenyl-Β-D-Galactopyranoside

O-Nitrophenyl-Β-D-Galactopyranoside

Synonyms o-Nitrophenyl-b-D-galactoside; 2-Nitrophenyl-b-D-galactopyranoside; ONP-b-D-gal; ONPG

CAS Number: 369-07-3 Molecular Formula: C12H15NO8

Beilstein Registry Number: 92207 EC Number: 206-716-1 MDL Number: MFCD00063255

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SKU 02102473-CF
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Description

Product Description

O-Nitrophenyl-Β-D-Galactopyranoside

Application Notes

o-Nitrophenyl-β-D-galactoside is a cell culture component for molecular genetics. It is chromogenic substrate for b-galactosidase and hence is useful in the detection of lacZ activity.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Substrate for β-galactosidase

Specifications
SKU 02102473-CF
Alternate Names o-Nitrophenyl-b-D-galactoside; 2-Nitrophenyl-b-D-galactopyranoside; ONP-b-D-gal; ONPG
Application Notes o-Nitrophenyl-β-D-galactoside is a cell culture component for molecular genetics. It is chromogenic substrate for b-galactosidase and hence is useful in the detection of lacZ activity.
Beilstein Registry Number 92207
Biochemical Physiological Actions β-galactosidase, breaks down lactose into galactose and glucose. β-Galactosidase is not lactose specific and can act on simple galactosides. 2-Nitrophenyl β-D-galactopyranoside hydrolysis results in the release of galactose and a yellow chromogenic compound. The test substrate does not depend on an induced or constitutive permease enzyme to enter the cell, therefore reactions are rapid and occur within a 24-hour period.
EC Number 206-716-1
Format Crystalline
Applications Substrate for β-galactosidase
MDL # MFCD00063255
Molecular Formula C12H15NO8
Optical Rotation -66 ° ± 7 ° (c=1, H2O)
Personal Protective Equipment Eyeshields, Gloves, respirator filter
Physical Appearance White Solid
Product Families Description o-Nitrophenyl-β-D-galactopyranoside
Product Overview O-Nitrophenyl-Β-D-Galactopyranoside
Storage and Handling Store at + 4 °C.
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
UV Visible Absorbance 262 ± 3 nm λ max (H2O)