Dino

Hepes

Hepes

Synonyms 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid; N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)

CAS Number: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight: 238.302 g/mol

Beilstein Registry Number: 883043 EC Number: 230-907-9 MDL Number: MFCD00006158

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SKU 02101926-CF
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Description

Product Description

Hepes

Application Notes

HEPES has been utilized extensively for research and commercial applications in the cell culture and tissue culture industries. HEPES is widely used in cell culture, largely because it is better at maintaining physiological pH despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture.Its dissociation decreases as the temperature decreases which makes it a more effective buffering agent for maintaining enzyme structure and function at low temperatures.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Buffer

Specifications
SKU 02101926-CF
Application Notes HEPES has been utilized extensively for research and commercial applications in the cell culture and tissue culture industries. HEPES is widely used in cell culture, largely because it is better at maintaining physiological pH despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture.Its dissociation decreases as the temperature decreases which makes it a more effective buffering agent for maintaining enzyme structure and function at low temperatures.
Beilstein Registry Number 883043
EC Number 230-907-9
Format Crystalline; Free Acid
Hazard Statements H317
Applications Buffer
Moisture Content <1.0%
Molecular Formula C8H18N2O4S
Molecular Weight 238.302 g/mol
Personal Protective Equipment Eyeshields, Gloves, Respirator filter
Physical Appearance White Powder
pKa 7.55 at 25 °C (Lit.)
Product Families Description HEPES
Product Overview Hepes
Purity ≥98%
RTECS Number TL6809000
Solubility 5.25e+01 g/l
Storage and Handling Store at Room Temperature (15-30 °C). Store Desiccated.
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
UV Visible Absorbance OD250 (10% aq soln) <0.2 , OD260 (10% aq soln) <0.1 and OD280 (10% aq soln) <0.1