Trypsin

Trypsin

Synonyms Parenzymol; Parenzyme; Tryptar; Trypure; U-4858; Tryptase; Cocoonase; Parenzyme

CAS Number: 2594141

EC Number: 232-650-8 MDL Number: MFCD00082094

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SKU 02101179-CF
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Description

Product Description

Trypsin

Application Notes

Trypsin can be used to dissolve blood clots in its microbial form and treat inflammation in its pancreatic form. Trypsin is commonly used in biological research.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Dissolve blood clots

Specifications
SKU 02101179-CF
Alternate Names Parenzymol; Parenzyme; Tryptar; Trypure; U-4858; Tryptase; Cocoonase; Parenzyme
Application Notes Trypsin can be used to dissolve blood clots in its microbial form and treat inflammation in its pancreatic form. Trypsin is commonly used in biological research.
Biochemical Physiological Actions Trypsin cleaves peptides on the C-terminal side of lysine and arginine residues. The rate of hydrolysis of this reaction is slowed if an acidic residue is on either side of the cleavage site and hydrolysis is stopped if a proline residue is on the carboxyl side of the cleavage site. Trypsin can also act to cleave ester and amide linkages of synthetic derivatives of amino acids. EDTA is added to trypsin solutions as a chelating agent that neutralizes calcium and magnesium ions that obscure the peptide bonds on which trypsin acts. Removing these ions increases the enzymatic activity.
Components Trypsin is composed of two subunits, α-trypsin and β-trypsin. α-Trypsin is composed of two peptide chains and β-trypsin is composed of one chain.
EC Number 232-650-8
Extinction Coefficient E1%280 = 14.3 (Lit.)
Format lyophilized powder
Grade 2× Crystallized
Hazard Statements H315-H319-H334-H335
Isoelectric Point pH 10.5(Lit.)
Applications Dissolve blood clots
MDL # MFCD00082094
Personal Protective Equipment Dust mask , Eyeshields, Faceshields, Gloves
Physical Appearance White Powder
Product Families Description Trypsin
Product Overview Trypsin
RTECS Number YN5075000
Safety Symbol GHS07, GHS08
Source Beef pancreas
Specific Activity ≥180 TAME u/mg
Specificity The protease activity of trypsin is highly specific toward positively charged side chains with lysine and arginine. Forms complexes with a2-macroglobulin. Can be used in the isolation of intact; detergent-free phycobilisomes and in the hydrolysis/condensation of carboxylic ester bonds.
Storage and Handling +4 °C
Typical Working Concentration For the Removal of Adherent Cells from a Culture Surface: 2.5% w/v solution
Unit Definition One unit hydrolyzes one µmole of TAME per minute at 25 °C, pH 8.2, in the presence of ionized calcium.
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.