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Trypsin Inhibitor

Trypsin Inhibitor

Synonyms Kunitz Trypsin Inhibitor; Tia1; STI; SBT1

CAS Number: 9035-81-8

EC Number: 232-906-9 MDL Number: MFCD00082103

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SKU 02101113-CF
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$44.20
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Description

Product Description

Trypsin Inhibitor

Application Notes

After trypsinizing cells, resuspend cells in 1 mL trypsin inhibitor solution (1mg/ml) for every mL of trypsin solution used for dissociation. Centrifuge the cell suspension at 1000 rpm for 5 minutes. A cell pellet should form. Remove as much of the trypsin inhibitor solution as possible and resuspend the pellet in serum-free medium. Culture cells as desired.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Cell culture

Specifications
SKU 02101113-CF
Application Notes After trypsinizing cells, resuspend cells in 1 mL trypsin inhibitor solution (1mg/ml) for every mL of trypsin solution used for dissociation. Centrifuge the cell suspension at 1000 rpm for 5 minutes. A cell pellet should form. Remove as much of the trypsin inhibitor solution as possible and resuspend the pellet in serum-free medium. Culture cells as desired.
Biochemical Physiological Actions Soybean trypsin inhibitor inhibits trypsin and to a lesser extent chymotrypsin and plasmin. It forms a 1:1 stoichiometric complex with trypsin. Upon formation of this complex, trypsin may cleave a single arginine-isoleucine bond in the inhibitor. Dissociation of this complex may yield the modified form or the native inhibitor. At the optimal pH for trypsin binding (pH 8.0), the association constant is ≥ 10x108.
EC Number 232-906-9
Extinction Coefficient E1% = 9.94 (280 nm, pH 7.6 buffer) (Lit.)
Format powder
Hazard Statements H317-H334
Isoelectric Point 4.5 (Lit.)
Applications Cell culture
Personal Protective Equipment Eyeshields, Gloves, respirator filter
Physical Appearance Tan Powder
Product Families Description Trypsin inhibitor
Product Overview Trypsin Inhibitor
Safety Symbol GHS08
Source Soybean
Specific Activity ≥10,000 BAEE units of inhibition/mg material
Storage and Handling Store at -20 °C; desiccate.
Unit Definition One unit will inhibit one trypsin BAEE unit. One trypsin unit causes an increase of absorbance at 253 nm of 0.001 per minute at 25 °C.
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.