RIBONUCLEASE A (BOVINE

RIBONUCLEASE A (BOVINE

Synonyms Pancreatic Ribonuclease; RNase A; Ribonuclease I; Ribonucleate 3′ pyrimidinooligonucleotidohydrolase

CAS Number: 9001-99-4 Molecular Weight: 13700 (based on Amino acid sequence)

EC Number: 232-646-6 MDL Number: MFCD00132181

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SKU 02101076-CF
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Specifications
SKU 02101076-CF
Application Notes Ribonuclease A, and to a greater extent its oligomers and some homologs (such as onconase from frogs), have cytotoxic and cytostatic effects, particularly on cancer cells.This attribute has led to the development of onconase as a cancer therapeutic.
Biochemical Physiological Actions Ribonuclease A is an endoribonuclease that cleaves single stranded RNA after pyrimidine nucleotides. It attacks at the 3’ phosphate end. Ribonucleases do not hydrolyze DNA, because the DNA lacks 2'-OH groups essential for the formation of cyclic intermediates. RNase can also hydrolyze RNA from protein samples. RNase A can be inhibited by alkylation of His12 and His119 and activated by potassium and sodium salts.
EC Number 232-646-6
Extinction Coefficient 7.3 (280 nm) (Lit.)
Isoelectric Point pH 9.45 (Lit.)
Applications Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. It is also used in RNA sequence analysis and protection assays.
Molecular Weight 13700 (based on Amino acid sequence)
Personal Protective Equipment Eyeshields, Gloves, Repirator Filter
pH Optimum pH: 7.0 - 7.5
Physical Appearance White Lyophilized Powder
RTECS Number RF0760000
Solubility Dissolves readily at 2 mg/mL in analytical grade water to give a clear colorless solution.
Source Bovine Pancreas
Specific Activity ≥50 Kunitz u/mg
Specificity The enzyme is specific for pyrimidine nucleoside linkages; i.e.; those cleaving cyclic 2';3'-pyrimidine nucleotide residues either singly or at the termination of purine nucleotide chains.
Storage and Handling Store at -0 °C;dessicate.
Unit Definition That amount of enzyme causing the hydrolysis of RNA at a rate such that k (velocity constant) equals unity at 25 °C and pH 5.0.
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.