Lysozyme

Lysozyme

Synonyms Muramidase; Mucopeptide N-acetylmuramoylhydrolase

CAS Number: 9001-63-2 Molecular Weight: 2235.597 g/mol

EC Number: 235-747-3 MDL Number: MFCD00131557

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SKU 02100834-CF
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Description

Product Description

Lysozyme

Application Notes

Lysozyme has been used for lysing E. coli and Streptomycetes for extraction purposes such as extracting group specific antigen. It would appear that lysozyme may act as a germinative agent of bacterial spores. The enzyme is often used for lysing bacterial cells by hydrolyzing the peptidoglycan present in the cell walls. Used to prepare spheroplasts.

Usage Statement

Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.

Key Applications

Germinative agent||Extracting group specific antigen

Specifications
SKU 02100834-CF
Alternate Names Muramidase; Mucopeptide N-acetylmuramoylhydrolase
Application Notes Lysozyme has been used for lysing E. coli and Streptomycetes for extraction purposes such as extracting group specific antigen. It would appear that lysozyme may act as a germinative agent of bacterial spores. The enzyme is often used for lysing bacterial cells by hydrolyzing the peptidoglycan present in the cell walls. Used to prepare spheroplasts.
Biochemical Physiological Actions Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane.
EC Number 235-747-3
Extinction Coefficient 26.4 (Lit.)
Format Crystalline powder
Gene ID LYZ(396218)
Hazard Statements H317
Isoelectric Point 11.0(Lit.)
Applications Germinative agent||Extracting group specific antigen
MDL # MFCD00131557
Molecular Weight 2235.597 g/mol
Personal Protective Equipment Eyeshields, Gloves, respirator filter
Physical Appearance Off-white Crystalline Powder
Product Families Description Lysozyme
Product Overview Lysozyme
RTECS Number OL5987000
Source Chicken eggwhite
Specific Activity ≥9000 Shugar µ/mg solid
Storage and Handling -20 °C.
Unit Definition One unit will cause a decrease in OD450 of 0.001 per minute at pH 6.24 and 25 °C using Micrococcus lysodeikticus as substrate.
Usage Statement Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.