Lysozyme

Lysozyme

Synonyms Muramidase; Mucopeptide N-acetylmuramoylhydrolase

CAS Number: 9001-63-2 Molecular Weight: 2235.597 g/mol

EC Number: 232-620-4 MDL Number: MFCD00131557

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SKU 02100831-CF
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Description

Product Description

Lysozyme

Application Notes

Lysozyme has been used for lysing E. coli and Streptomycetes for extraction purposes such as extracting group specific antigen. It would appear that lysozyme may act as a germinative agent of bacterial spores.In humans, lysozyme may be the mediator in the anti-tumor function of macrophages which, it has been shown, secrete the enzyme. There is evidence that cartilage lysozyme has a role in cartilage calcification. Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts.

Usage Statement

Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.

Key Applications

Cell Lysis

Specifications
SKU 02100831-CF
Alternate Names Muramidase; Mucopeptide N-acetylmuramoylhydrolase
Application Notes Lysozyme has been used for lysing E. coli and Streptomycetes for extraction purposes such as extracting group specific antigen. It would appear that lysozyme may act as a germinative agent of bacterial spores.In humans, lysozyme may be the mediator in the anti-tumor function of macrophages which, it has been shown, secrete the enzyme. There is evidence that cartilage lysozyme has a role in cartilage calcification. Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts.
Biochemical Physiological Actions Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane. The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).
EC Number 232-620-4
Extinction Coefficient 26.4 (280 nm) (Lit.)
Format 3X Crystallized; Salt-Free; Albumin-Free; Lyophilized
Isoelectric Point pH 11.0 (lit)
Applications Cell Lysis
MDL # MFCD00131557
Molecular Weight 2235.597 g/mol
Personal Protective Equipment Eyeshields, Gloves, respirator filter
Physical Appearance White powder
Product Families Description Lysozyme
Product Overview Lysozyme
RTECS Number OL5987000
Source Chicken eggwhite
Specific Activity ≥20,000 u/mg solid
Storage and Handling -20°C
Unit Definition One unit will cause a decrease in A450 of 0.001 per minute at pH 6.24 and 25 °C using Micrococcus lysodeikticus as substrate.
Usage Statement Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.