Elastase

Elastase

Synonyms Elastase from hog pancreas; Pancreatopeptidase E

CAS Number: 2594837

EC Number: 254-453-6 MDL Number: MFCD00130998

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SKU 02100617-CF
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Description

Product Description

Elastase

Application Notes

Elastase was used to study human emphysema by induction of emphysema in rodents. It has been used in a study to investigate the design, synthesis and evaluation of biomimetic affinity ligands for elastases. Elastase from porcine pancreas has also been used in a study to investigate the purification and partial characterization of the pancreatic proteolytic enzymes trypsin, chymotrypsin, and elastase. It hydrolyzes peptide bonds, especially those adjacent to neutral amino acids.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Investigate the purification and partial characterization of the pancreatic proteolytic enzymes

Specifications
SKU 02100617-CF
Alternate Names Elastase from hog pancreas; Pancreatopeptidase E
Application Notes Elastase was used to study human emphysema by induction of emphysema in rodents. It has been used in a study to investigate the design, synthesis and evaluation of biomimetic affinity ligands for elastases. Elastase from porcine pancreas has also been used in a study to investigate the purification and partial characterization of the pancreatic proteolytic enzymes trypsin, chymotrypsin, and elastase. It hydrolyzes peptide bonds, especially those adjacent to neutral amino acids.
Biochemical Physiological Actions Elastase hydrolyses elastin, the specific protein of elastic fibers, and digests hemoglobin, casein and fibrin.
EC Number 254-453-6
Format solution
Formulation Details Aqueous suspension with 0.01% sodium azide as preservative
Grade 2x CRYSTALLIZED
Hazard Statements H315-H319-H335
Applications Investigate the purification and partial characterization of the pancreatic proteolytic enzymes
MDL # MFCD00130998
Personal Protective Equipment Dust mask, Eyeshields, Faceshields, Gloves
Physical Appearance Milky Solution
Preparation Method Elastase is prepared from porcine pancreas. It is lyophilized and water soluble. Elastase is chromatographically prepared by the method of Narayanan and Anwar. In the method, 2 times crystalline elastase is adsorbed on a column of DEAE-Sephadex A50 to separate elastase and non-specific protein components. The elastase component is further purified by chromatography on a column of CM-Cellulose using a sodium chloride gradient to elute the elastase. The latter is dialyzed until chloride-free and then lyophilized. During its preparation the elastase is held below a pH of 5.5 for greater than 24 hours. Two times crystallized from the euglobin fraction of porcine pancreas by the method of Lewis et al. Does not contain trypsin or chymotrypsin.
Product Families Description Elastase
Product Overview Elastase
RTECS Number JX7069000
Safety Symbol GHS07
Source Porcine pancreas
Specific Activity Activity ≥50 u/mg protein
Storage and Handling +4 °C
Unit Definition One unit will solublize 1 mg of elastin in 20 minutes at 37 °C, pH 8.8.
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.