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Dl-Dithiothreitol

Dl-Dithiothreitol

Synonyms 1,4-Dithiothreitol; DTT; 1,4-Dithio-DL-threitol; Cleland Reagent; (±)-threo-1,4-Dimercapto-2,3-butanediol; Cleland’s reagent

CAS Number: 578517 Molecular Formula: C4H10O2S2

Beilstein Registry Number: 1719757 EC Number: 248-531-9 MDL Number: MFCD00004877

SKU 02100597-CF
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Description

Product Description

Dl-Dithiothreitol

Application Notes

Dithiothreitol is useful for stabilizing sulfhydryl containing enzymes. Effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N'-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).DTT has been used:• as one of the reactants in the reduction and alkylation of as1-Casein, the major allergen of cow's milk.• as a component of medium for the demembranation and reactivation of spermatozoa.• to maintain stability of the enzyme as thiol effectively protects the active sites of the biocatalyst.• as a reducing agent to test the specificity of the reaction of N-Ethylmaleimide with sulfhydryl groups.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Sulfhydryl reducing agent

Specifications
SKU 02100597-CF
Application Notes Dithiothreitol is useful for stabilizing sulfhydryl containing enzymes. Effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N'-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).DTT has been used:• as one of the reactants in the reduction and alkylation of as1-Casein, the major allergen of cow's milk.• as a component of medium for the demembranation and reactivation of spermatozoa.• to maintain stability of the enzyme as thiol effectively protects the active sites of the biocatalyst.• as a reducing agent to test the specificity of the reaction of N-Ethylmaleimide with sulfhydryl groups.
Beilstein Registry Number 1719757
EC Number 248-531-9
Format powder
Hazard Statements H302-H315-H319-H335
Applications Sulfhydryl reducing agent
Molecular Formula C4H10O2S2
Personal Protective Equipment Dust mask, Eyeshields, Faceshields, Gloves
Physical Appearance White Powder
Product Families Description Dithiothreitol (DTT)
Product Overview Dl-Dithiothreitol
Purity ≥99.0%
RTECS Number XO8576500
Safety Symbol GHS07
Storage and Handling +4°C
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.