Dino

Deoxyribonuclease I

Deoxyribonuclease I

Synonyms DNase I; Deoxyribonucleate 5′-oligonucleotido-hydrolase

CAS Number: 9003-98-9

EC Number: 232-667-0 MDL Number: MFCD00130918

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SKU 02100575-CF
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Description

Product Description

Deoxyribonuclease I

Application Notes

DNase I is used as a genetic marker for forensic identification. Used for the removal of DNA from protein samples. DNAse I is used to nick DNA, as a first step to incorporate labeled bases into DNA. The enzyme from Sigma has been used in the nuclease stock along with RNAse during cell lysate preparation of Madin-Darby canine kidney (MDCK) II cell lines. It has also been used during RNA extraction from Sindbis virus. Deoxyribonuclease I from bovine pancreas has been used for the identification, localization and expression of two novel human genes similar to deoxyribonuclease I. Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate a new approach to obtaining high-activity RNase, DNase, cholesterolesterase, and trypsin from cattle pancreas.

Usage Statement

Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.

Key Applications

Marker||Cell lysis

Specifications
SKU 02100575-CF
Application Notes DNase I is used as a genetic marker for forensic identification. Used for the removal of DNA from protein samples. DNAse I is used to nick DNA, as a first step to incorporate labeled bases into DNA. The enzyme from Sigma has been used in the nuclease stock along with RNAse during cell lysate preparation of Madin-Darby canine kidney (MDCK) II cell lines. It has also been used during RNA extraction from Sindbis virus. Deoxyribonuclease I from bovine pancreas has been used for the identification, localization and expression of two novel human genes similar to deoxyribonuclease I. Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate a new approach to obtaining high-activity RNase, DNase, cholesterolesterase, and trypsin from cattle pancreas.
Biochemical Physiological Actions DNase I is an endonuclease that acts on phosphodiester bonds adjacent to pyrimidines to produce polynucleotides with terminal 5'-phosphates. In the presence of Mg2+, DNAse I cleaves each strand of DNA independently and the cleavage sites are random. Both DNA strands are cleaved at approximately the same site in the presence of Mn2+. The pH optimum is found to be between 7 and 8. Divalent cations such as Mn2+, Ca2+, Co2+, and Zn2+ are activators of the enzyme. A concentration of 5 mM Ca2+ stabilizes the enzyme against proteolytic digestion. DNAse I from bovine pancreas consists of four chromatographically distinguishable components, A, B, C, and D, with their molar ratios being 4:1:1 respectively. Only minor amounts of D are found. 2-Mercaptoethanol, chelators, sodium dodecyl sulfate (SDS) and actin are known to inhibit the enzyme activity.
EC Number 232-667-0
Extinction Coefficient E1%280= 11.1.
Formulation Details A lyophilized powder containing a small amount of glycine-stabilizer
Applications Marker||Cell lysis
Pack Size No
Physical Appearance White powder
Product Families Description Deoxyribonuclease I
Product Overview Deoxyribonuclease I
RTECS Number RF0750000
Source Bovine Pancreas
Specific Activity ≥2000 kunitz u/mg dw
Storage and Handling Store at +4 °C; desiccate.
Unit Definition One unit causes an increase in absorbance at 260nm of 0.001/minute per ml at 25°C, pH 5.0, when acting on highly polymerized DNA in the presence of ionized magnesium and calcium.
Usage Statement Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.