Puromycin Dihydrochloride

Puromycin Dihydrochloride

Synonyms 6-dimethylamino-9-(3'-deoxy-3'-(p-methoxy-L-phenylalanyamino)- B-D-ribofuranosyl)-purine

CAS Number: 58-58-2 Molecular Formula: C22H29N7O5 · 2HCl Molecular Weight: 544.434 g/mol

Beilstein Registry Number: 3853613 EC Number: 200-387-8 MDL Number: MFCD00012691

Configure Your Product:

SKU 02100552-CF
Price:
$30.40
In Stock
Request a quote
Description

Product Description

Puromycin Dihydrochloride

Application Notes

Puromycin is Nucleoside antibiotic & protein synthesis inhibitor that causes premature chain termination by acting as an analog of the 3′-terminal end of aminoacyl-tRNA. Prevents growth of bacteria, protozoa, algae, and mammalian cells.Puromycin dihydrochloride is a part of the amino-nucleoside family of antibiotics and is derived from Streptomyces alboniger. It is a broad spectrum antibiotic with antitumor activity, as an inhibitor of protein synthesis and has been used to study transcription regulatory mechanisms that control the sequential and coordinate expression of genes during cell differentiation.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Antibiotic

Specifications
SKU 02100552-CF
Alternate Names 6-dimethylamino-9-(3'-deoxy-3'-(p-methoxy-L-phenylalanyamino)- B-D-ribofuranosyl)-purine
Application Notes Puromycin is Nucleoside antibiotic & protein synthesis inhibitor that causes premature chain termination by acting as an analog of the 3′-terminal end of aminoacyl-tRNA. Prevents growth of bacteria, protozoa, algae, and mammalian cells.Puromycin dihydrochloride is a part of the amino-nucleoside family of antibiotics and is derived from Streptomyces alboniger. It is a broad spectrum antibiotic with antitumor activity, as an inhibitor of protein synthesis and has been used to study transcription regulatory mechanisms that control the sequential and coordinate expression of genes during cell differentiation.
Beilstein Registry Number 3853613
Biochemical Physiological Actions Mode of Action: Puromycin inhibits protein synthesis by causing premature chain termination acting as an analog of the 3'-terminal end of the aminoacyl-tRNA.Mode of Resistance: Puromycin acetyltransferase is an effective resistance gene.Antimicrobial Spectrum: This product is active against gram-positive microorganisms, less active against acid-fast bacilli and more weakly active against gram-negative microorganisms. Puromycin can prevent growth of bacteria, protozoa, algae and mammalian cells and acts quickly, killing 99% of cells within 2 days.
EC Number 200-387-8
Extinction Coefficient 20.3 (275 nm, 0.1 N NaOH), 19.5 (268 nm, 0.1 NHCl) (Lit.)
Hazard Statements H302
Applications Antibiotic
MDL # MFCD00012691
Molecular Formula C22H29N7O5 · 2HCl
Molecular Weight 544.434 g/mol
Personal Protective Equipment Dust mask, Eyeshields, Gloves
Physical Appearance Off-white Powder
pKa 6.8, 7.2 (Lit.)
Product Families Description Puromycin dihydrochloride
Product Overview Puromycin Dihydrochloride
RTECS Number AU7355000
Safety Symbol GHS07
Solubility Soluble (NTP, 1992)
Source Streptomyces albo-niger
Storage and Handling -20 °C
Typical Working Concentration The recommended working concentration in eukaryotic cell culture is 1-10 µg/mL.
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
UV Visible Absorbance 268 ± 2 nm (λ max (0.1N HCl)