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P-Nitro Blue Tetrazolium Chloride

P-Nitro Blue Tetrazolium Chloride

Synonyms 2,2'-bis(4-Nitrophenyl)-5,5'-diphenyl-3,3'-(3,3'-dimethoxy-4,4'-diphenylene)ditetrazolium chloride; 3,3'-(3,3'-Dimethoxy-4,4'-biphenylene)bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride]; p-Nitro-Blue tetrazolium chloride; p-Nitrotetrazolium blue; NBT; Nitro BT

CAS Number: 298-83-9 Molecular Formula: C40H30CI2N10O6 Molecular Weight: 817.644 g/mol

Beilstein Registry Number: 4115923 EC Number: 206-067-4 MDL Number: MFCD00012159

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SKU 02100416-CF
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Description

Product Description

P-Nitro Blue Tetrazolium Chloride

Application Notes

p - nitro blue tetrazolium is used for the detection of alkaline phosphatase on western blots. It has also been used as a redox indicator for other enzymatic reactions including dehydrogenases, threonine deaminase, glucose-6-phosphate dehydrogenase, phosphofructokinase on polyacrylamide gels, oxidases on polyacrylamide gels, and pentose shunt dehydrogenases. Redox and halfwave potentials have been determined for p - nitro blue tetrazolium. It has also been used as a colorimetric indicator of bacterial infection in blood samples.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Colorimetric detection of alkaline phosphatase conjugates

Specifications
SKU 02100416-CF
Application Notes p - nitro blue tetrazolium is used for the detection of alkaline phosphatase on western blots. It has also been used as a redox indicator for other enzymatic reactions including dehydrogenases, threonine deaminase, glucose-6-phosphate dehydrogenase, phosphofructokinase on polyacrylamide gels, oxidases on polyacrylamide gels, and pentose shunt dehydrogenases. Redox and halfwave potentials have been determined for p - nitro blue tetrazolium. It has also been used as a colorimetric indicator of bacterial infection in blood samples.
Beilstein Registry Number 4115923
EC Number 206-067-4
Extinction Coefficient 260 nm: E1% = 740 (in water) (Lit.)
Format powder
Hazard Statements H302-H370
Applications Colorimetric detection of alkaline phosphatase conjugates
Molecular Formula C40H30CI2N10O6
Molecular Weight 817.644 g/mol
Personal Protective Equipment Eyeshields, Gloves, respirator filter
Physical Appearance Yellow Powder
Product Families Description p-Nitro-Blue-tetrazolium chloride
Product Overview P-Nitro Blue Tetrazolium Chloride
RTECS Number XF8045000
Safety Symbol GHS07, GHS08
Storage and Handling Store at + 4 °C.
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
UV Visible Absorbance 255 ± 3 nm ( λ max (MeOH))