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PeroxySafe test kits are designed to provide rapid analysis of lipid peroxides in any type of oil, food, or animal matrix using microanalytical and membrane separation principles. Lipid peroxides are the primary product of oxidized oils or fats and are an indicator of off flavors and off odors (rancidity). The practical dynamic range of the determination has been established as 0.02 to 50 meq/kg.

  • Peroxide Value Overview
  • PeroxySafe Kits
  • Lipid peroxide Analysis
  • PeroxySafe Protocols
Lipid Peroxide Analysis with PeroxySafe Kits


What are Lipid Peroxides?

Lipid peroxides are the primary product of oxidized oils or fats. Virtually anything in this world can oxidize. What does oxidation mean? Oxidation can be thought of as "rusting". When metal oxidizes we get rust. When fats and oils oxidize, we get lipid peroxides. Oxidation is the loss of electrons from atoms or molecules. When a molecule loses its electrons, it becomes a new and different type of molecule. Phospholipids, a typical lipid in animals and plants, is a major target for lipid oxidation due to its increased surface area and higher degree of unsaturation. When lipids or phospholipids are exposed to air they react with oxygen. Oxygen is very electro-negative, so it likes to grab positively charged atoms or molecules like hydrogen, leaving other atoms or molecules with their electrons exposed. Compounds with two adjacent oxygens and no hydgrogens or other positively charged atoms to couple to the exposed electrons are called peroxides. The peroxide formation accelerates formation of other peroxides in a propagation step. Peroxides are referred to as free radicals. There has been much emphasis in the press and from the health industry on preventing free radicals. Free radicals, or peroxides will destroy the normal state of many chemicals in our bodies, in food products, and other biological matter. In food, the presence of peroxides will immediately alter the taste. Once oxidation has run its course, the oxidized material will have essentially changed to a new substance. The new substance is chemically different from its original form and potentially toxic, which is why it is considered rancid and unusable.


What are PeroxySafe?

The PeroxySafe Kit is designed to provide rapid analysis of lipid peroxides in any type of oil, food or animal matrix using micro-analytical and membrane separation principles. The PeroxySafe Kit quantitates peroxide value (PV) by transferring a free electron to a metal-chromogen complex whose visible spectrum then changes and is read using the SafTest analyzer.

The PeroxySafe Kit was evaluated against the American Oil Chemists' Society (AOCS) Official Method Cd 8-53, commonly used in food testing laboratories. The AOCS method requires large volumes of organic solvents, large sample aliquots, and it involves a lengthy titration process, as well as extraction for some samples which can take several hours. The proprietary PeroxySafe Kit can be used for testing solubilized dry and wet food matrices by extracting lipid fat from the sample matrix using our preparation reagent and employing mechanical mixing and warming techniques. This results in significantly shorter sample preparation (15 minutes) allowing for data to be obtained in under 30 minutes. The PeroxySafe Kit contains all reagents and calibrators required for testing up to 120 tests. The PV value is expressed as milliequivalents of peroxide per kilogram of sample.


Sample Preparation: Liquid oils may require heating or dilution
Semi-solid/solid oils and meals require heating and mechanical disruption
Meals require membrane separation
Time Requirement: Sample Preparation 5 to 20 minutes
Analysis time approximately 10-15 minutes
Limit of Qualification: 0.02 to 50 meq/kg
PeroxySafe Kits


The PeroxySafe MSA Kit is designed to provide rapid analysis of peroxide value of oils, tallows, greases, feed, and protein meals using micro-analytical and membrane separation principles. The PeroxySafe MSA kit quantitates peroxide value (PV) by using a pH sensitive chromogen whose visible spectrum then changes and is read using the SafTest Analyzer. The PeroxySafe MSA Kit was evaluated against the American Oil Chemists' Society (AOCS) Official Method Cd 8-53 method, commonly used in food testing laboratories. The conventional method requires large volumes of organic solvents, large sample aliquots, and it involves a lengthy titration process, as well as extraction for some samples which can take several hours. The proprietary PeroxySafe MSA Kit can be used for testing solubilized dry and wet food matrices by extracting lipid fat from the sample matrix using our preparation reagent and employing mechanical mixing and warming techniques. The significantly shorter sample preparation of 15 to 20 minutes permits data to be obtained in 25 to 30 minutes. MP Biomedicals' PeroxySafe MSA Kit contains the reagents and calibrators required for testing up to 120 tests. The peroxide value is expressed as milliequivalents of peroxide per kilogram of fat.



The PeroxySafe STD Kit is designed to provide rapid analysis of peroxide value of oils, tallows, greases, feed, and protein meals using micro-analytical and membrane separation principles. The PeroxySafe STD kit quantitates peroxide value (PV) by using a pH sensitive chromogen whose visible spectrum then changes and is read using the SafTest Analyzer. The PeroxySafe STD Kit was evaluated against the American Oil Chemists' Society (AOCS) Official Method Cd 8-53 method, commonly used in food testing laboratories. The conventional method requires large volumes of organic solvents, large sample aliquots, and it involves a lengthy titration process, as well as extraction for some samples which can take several hours. The proprietary PeroxySafe STD Kit can be used for testing solubilized dry and wet food matrices by extracting lipid fat from the sample matrix using our preparation reagent and employing mechanical mixing and warming techniques. The significantly shorter sample preparation of 15 to 20 minutes permits data to be obtained in 25 to 30 minutes. MP Biomedicals' PeroxySafe STD Kit contains the reagents and calibrators required for testing up to 120 tests. The peroxide value is expressed as milliequivalents of peroxide per kilogram of fat.



The PeroxySafe HSY Kit is designed to provide rapid analysis of peroxide value of oils, tallows, greases, feed, and protein meals using micro-analytical and membrane separation principles. The PeroxySafe HSY kit quantitates peroxide value (PV) by using a pH sensitive chromogen whose visible spectrum then changes and is read using the SafTest Analyzer. The PeroxySafe HSY Kit was evaluated against the American Oil Chemists' Society (AOCS) Official Method Cd 8-53 method, commonly used in food testing laboratories. The conventional method requires large volumes of organic solvents, large sample aliquots, and it involves a lengthy titration process, as well as extraction for some samples which can take several hours. The proprietary PeroxySafe HSY Kit can be used for testing solubilized dry and wet food matrices by extracting lipid fat from the sample matrix using our preparation reagent and employing mechanical mixing and warming techniques. The significantly shorter sample preparation of 15 to 20 minutes permits data to be obtained in 25 to 30 minutes. MP Biomedicals' PeroxySafe HSY Kit contains the reagents and calibrators required for testing up to 120 tests. The peroxide value is expressed as milliequivalents of peroxide per kilogram of fat.

Lipid Peroxide Analysis with PeroxySafe Kits

AOAC Certification# 030501

Methodology

To demonstrate method repeatability, accuracy and linearity, vegetable and marine (fish) oil samples, oil and seed oils, and meals and protein powders were sent to an independent method validation laboratory for validation. Samples were analyzed using the AOCS Official Method Cd 8-53 and SafTest PeroxySafe Kit.


Vegetable oils, seed oils, marine oils, olive oils, pressed oils

Approximately 220 samples of various types of vegetable, seed and marine oils were analyzed. Excellent agreement (i.e. correlation coefficient, r, 0.97 to 0.99) observed from method-to-method comparison of PeroxySafe and the traditional AOCS method for all oils.

Meals and protein powders

One hundred samples of 10 food categories of meals and protein powders were extracted and analyzed by the traditional AOCS method and using membrane separation using PeroxySafe. Excellent agreement (e.g. correlation coefficient, r, of 0.99) and linear relationship was observed between the two methods. This data validates the useful range of PeroxySafe from 0.2-10.0 meq PV/kg. Furthermore, the mean of 20 individual %RSDs (%CV) values yielded an estimate of the within-laboratory precision (repeatability) of 6% for meals.


Animal fats and oils

A strong correlation (e.g. correlation coefficient, r, of 0.99) and a linear relationship were observed between the data generated using the PeroxySafe and the AOCS method for 100 samples of animal fats and oils. The mean of 20 individual %RSDs (%CV) yielded an estimate of the within-laboratory precision (repeatability) of 5 percent for animal fats and oils.

Summary of Validated Claims

The PeroxySafe Kit is designed to measure milliequivalents (meq) of peroxide per kilogram (kg) of sample of oils and fats in raw and finished products. The dynamic range of determination is 0.01 to 5.0 milliequivalents per kilogram (meq/kg) in these matrices and four times these levels for solid matrix samples. SafTest has demonstrated that the PeroxySafe Kit is capable of analyzing a wide range of triglycerides with a high degree of accuracy and precision.


Sensitivity: Determination of Limit of Detection and Limit of Quantitation

The LOD has been empirically determined as 3x SD of 10 replicate analyses of a low control and is calculated as 0.005 meq/kg of sample. The empirical detection limit study has demonstrated that this method is capable of measuring peroxides as low as 0.02-meq/kg with a high degree of confidence.


Repeatability and Accuracy Studies

Strong agreement was observed (correlation coefficient, r, of 0.99 and 0.97) between the results generated using PeroxySafe and the traditional AOCS method on 40 meal samples and 276 different types of oil samples.


Lot-to-Lot Reagent Stability (Ruggedness) Study

In all cases, pooled RSD (CV) values were well below 10% indicating close agreement (precision) between each set of results generated using the PeroxySafe Kit. Variations observed between three lots of chemicals prepared at different times were insignificant.

SafTest PeroxySafe Protocols






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