Instruments and Consumables

Testing Types and Kits

Need Your Sample Tested?

Customer Testimonials

Why Choose SafTest™?
Certifications
Reagents meet the rigorous standards established by AOAC and ASTM
Proven
Reagent kits are reproducible, lot-to-lot consistency, scalability, and easy to use
Speed
Evaluate 20 samples per hour, with as little as 10 - 50 μL sample volume
Convenience
Each kit is configured and standardized for analysis of 110 samples at a cost of $4.5 per sample
Safety
Reagents are low hazard and require no toxic chemicals or solvents

SafTest™ Training

FaSafe and AciSafe test kits are designed to measure free fatty acid (FFA) content in fresh and used oils and fats and in raw and finished products. Free Fatty Acids are a key indicator of hydrolytic degradation associated with off flavor and textural changes. The FFA is quantitated as percent oleic acid using an indicator that responds to the acids in the sample matrices. The practical dynamic range of the determination has been established as 0.3 to 20% oleic acid. However, samples below 0.3% oleic acid must be tested using the High Sensitivity Kit (FaSafe HSY Kit).

  • Free Fatty Acids Overview
  • FaSafe/AciSafe Kits
  • Free Fatty Acid Analysis
  • FaSafe/AciSafe Protocol
Free Fatty Acid Analysis with FaSafe/AciSafe Kits


What are Free Fatty Acids?

Fatty acids are the building blocks of fat sources in living organisms. Fat, or lipids are made up of 3 (fatty acids) attached to a glycerol backbone to make up a triglyceride. Since fatty acids are necessary to create essential building blocks such as triglycerides, they are rarely found floating alone within cells. When these acids are floating alone, they are referred to as free fatty acids. Free fatty acids appear as lipids breakdown and are therefore good indicators of degradation. There are many types of free fatty acids. They can be differentiated by the length of the carbon chain, the presence and number of double bonds and the alignment of the carbons at the double bonds (cis- and trans- fatty acid). Free fatty acids have been shown to have both beneficial and adverse effects on health. There is a great need in the food and cosmetic industries to have the ability to detect free fatty acids.


What is FaSafe/AciSafe?

The FaSafe and AciSafe Kits are designed to provide rapid analysis of the free fatty acid content of oils, tallows, greases, feed, and protein meals using micro-analytical and membrane separation principles. The FaSafe and AciSafe quantitates free fatty acids (FFA) by using a pH sensitive chromogen whose visible spectrum then changes and is read using the SafTest Analyzer. The FaSafe and AciSafe Kits were evaluated against the American Oil Chemists' Society (AOCS) Official Method Ca 5a-40 method, commonly used in food testing laboratories, which is based on titration of an ethanolic solution of the fat or oil material with ethanolic potassium hydroxide to a visually determined phenolphthalein endpoint. The conventional method requires large volumes of organic solvents, large sample aliquots, and it involves a lengthy titration process, as well as extraction for some samples which can take up to eighteen hours. The proprietary FaSafe and AciSafe Kits can be used for testing solubilized dry and wet food matrices by releasing lipid from the sample matrix using a stabilized reagent (i.e. stabilized isopropanol) and employing mechanical mixing and warming techniques. The solubilized food matrix is then filtered through a membrane and the filtrate is analyzed for free fatty acids. The significantly shorter sample preparation of 15 to 20 minutes permits data to be obtained in 25 to 30 minutes. MP Biomedicals' FaSafe and AciSafe kits contain the reagents, calibrators, and controls required for testing up to 120 samples. The Free Fatty Acid value is expressed as percent oleic acid.


Sample Preparation: Liquid oils may require heating or dilution; semi-solid/solid oils and meals require heating and mechanical disruption; meals require membrane separation
Time Requirement: Sample Preparation 5 to 20 minutes
Analysis time approximately 10 minutes
Limit of Quantitation: 0.3% oleic acid
Interferences: Highly colored samples, samples containing acids, bases, or chelating metal ions
FaSafe/AciSafe Kits


The AciSafe MSA Kit is designed to provide rapid analysis of the free fatty acid content of oils, tallows, greases, feed, and protein meals using micro-analytical and membrane separation principles. The AciSafe MSA kit quantitates free fatty acids (FFA) by using a pH sensitive chromogen whose visible spectrum then changes and is read using the SafTest Analyzer. The AciSafe Kit was evaluated against the American Oil Chemists' Society (AOCS) Official Method Ca 5a-40 method, commonly used in food testing laboratories, which is based on titration of an ethanolic solution of the fat or oil material with ethanolic potassium hydroxide to a visually determined phenolphthalein endpoint. The conventional method requires large volumes of organic solvents, large sample aliquots, and it involves a lengthy titration process, as well as extraction for some samples which can take up to eighteen hours. The proprietary AciSafe MSA Kit can be used for testing solubilized dry and wet food matrices by releasing lipid from the sample matrix using a stabilized reagent (i.e. stabilized isopropanol) and employing mechanical mixing and warming techniques. The solubilized food matrix is then filtered through a membrane and the filtrate is analyzed for free fatty acids. The significantly shorter sample preparation of 15 to 20 minutes permits data to be obtained in 25 to 30 minutes. MP Biomedicals' AciSafe MSA Kit contains the reagents and calibrators required for testing up to 120 tests. The Free Fatty Acid value is expressed as percent oleic acid.



The FaSafe STD Kit is designed to provide rapid analysis of the free fatty acid content of oils, tallows, greases, feed, and protein meals using micro-analytical and membrane separation principles. The FaSafe Kit quantitates free fatty acids (FFA) by using a pH sensitive chromogen whose visible spectrum then changes and is read using the SafTest Analyzer. The FaSafe STD Kit was evaluated against the American Oil Chemists' Society (AOCS) Official Method Ca 5a-40 method, commonly used in food testing laboratories, which is based on titration of an ethanolic solution of the fat or oil material with ethanolic potassium hydroxide to a visually determined phenolphthalein endpoint. The conventional method requires large volumes of organic solvents, large sample aliquots, and it involves a lengthy titration process, as well as extraction for some samples which can take up to eighteen hours. The proprietary FaSafe STD Kit can be used for testing solubilized dry and wet food matrices by releasing lipid from the sample matrix using a stabilized reagent (i.e. stabilized isopropanol) and employing mechanical mixing and warming techniques. The solubilized food matrix is then filtered through a membrane and the filtrate is analyzed for free fatty acids. The significantly shorter sample preparation of 15 to 20 minutes permits data to be obtained in 25 to 30 minutes. MP Biomedicals' FaSafe STD Kit contains the reagents, calibrators, and controls required for testing up to 120 tests. The Free Fatty Acid value is expressed as percent oleic acid.



The FaSafe HSY Kit is designed to provide rapid analysis of the free fatty acid content of oils, tallows, greases, feed, and protein meals using micro-analytical and membrane separation principles. The FaSafe HSY kit quantitates free fatty acids (FFA) by using a pH sensitive chromogen whose visible spectrum then changes and is read using the SafTest Analyzer. The FaSafe HSY Kit was evaluated against the American Oil Chemists' Society (AOCS) Official Method Ca 5a-40 method, commonly used in food testing laboratories, which is based on titration of an ethanolic solution of the fat or oil material with ethanolic potassium hydroxide to a visually determined phenolphthalein endpoint. The conventional method requires large volumes of organic solvents, large sample aliquots, and it involves a lengthy titration process, as well as extraction for some samples which can take up to eighteen hours. The proprietary FaSafe HSY Kit can be used for testing solubilized dry and wet food matrices by releasing lipid from the sample matrix using a stabilized reagent (i.e. stabilized isopropanol) and employing mechanical mixing and warming techniques. The solubilized food matrix is then filtered through a membrane and the filtrate is analyzed for free fatty acids. The shorter sample preparation time of 15 to 20 minutes permits data to be obtained in 25 to 30 minutes. MP Biomedicals' FaSafe HSY Kit contains the reagents, calibrators, and controls required for testing up to 120 tests. The Free Fatty Acid value is expressed as percent oleic acid.

The FaSafe HSY (high sensitivity) Kit differs from the AciSafe MSA and FaSafe STD Kits by detecting free fatty acids at a level of 0.3% oleic acid and below.

Free Fatty Acid Analysis with FaSafe and AciSafe Kits

AOAC Certification# 030405

Methodology

To demonstrate method repeatability, accuracy and linearity, vegetable and marine (fish) oil samples, oil and seed oils, and meals and protein powders were sent to an independent method validation laboratory for validation. Samples were analyzed using the AOCS Official method Ca 5a-40 and SafTest, FaSafe and AciSafe Kits.


Vegetable oils, seed oils, marine oils, olive oils, pressed oils

Approximately 120 samples of various types of vegetable, seed, and marine oils were analyzed. Close agreement (e.g. correlation coefficient, r, 0.98 to 0.93) observed from method-to-method comparison of FaSafe and AciSafe and the AOCS method for all oils.


Meals and protein powders

One hundred samples in 10 food categories of meals and protein powders were extracted and analyzed by the traditional AOCS method using membrane separation with FaSafe/AciSafe Kits. Excellent agreement (e.g. correlation coefficient, r, of 0.99) and linear relationship was observed between the two methods. This data validates the useful range of FaSafe/AciSafe from 0 to 23%. In addition, the mean of 20 individual RSD (CV) values yielded an estimate of the within-laboratory precision (repeatability) of 2.2% for meals.


Animal fats and oils

Excellent agreement (e.g. correlation coefficient, r, of 0.99) was observed between the data generated using the FaSafe and AciSafe Kits and the traditional AOCS method for 100 samples of animal fats and oils. Free fatty acid levels ranged from 1.0% to 13.0%. In addition, the mean of 20 individual RSDs (CV) yielded an estimate of the within-laboratory precision (repeatability) of 2.5% for animal fats and oils.



Summary of Validated Claims:

FaSafe and AciSafe test kits are designed to measure free fatty acid (FFA) content of fresh and used oils and fats and in raw and finished products. Free Fatty Acids are a key indicator of hydrolytic degradation associated with off flavor and textural changes. The FFA is quantitated as percent oleic acid using an indicator that responds to the acids in the sample matrices. The practical dynamic range of the determination has been established as 0.3% to 20% oleic acid. However, samples below 0.3% oleic acid must be tested using the High Sensitivity Kit (FaSafe HSY).


Sensitivity: Determination of Limit of Detection and Limit of Quantitation

The LOD for the FASafe and AciSafe Kits has been empirically determined as 3x SD of 10 replicate analyses of a low control and is calculated as 0.05% oleic acid. The empirical detection limit study has demonstrated that this method is capable of measuring FFA down to 0.3% with 95% confidence.


Repeatability and Accuracy Studies

Excellent correlation (correlation coefficient, r, of 0.99 and 0.98) was observed between the results generated using FaSafe and AciSafe Kits and the traditional AOCS method on 40 meal samples and 276 different types of oil samples, respectively.


Lot-to-Lot Reagent Stability (Ruggedness) Study

In all cases, pooled RSD (CV) values were well below 10% indicating close agreement (precision) between each set of results generated using the FaSafe and AciSafe Kits. Variations observed between three lots of chemicals prepared at different times were insignificant.

FaSafe/AciSafe Protocols






Supporting Technical Information

Browse SafTest's Technical Resource Center for all the technical resources needed to support your decision-making process.



Download the Current SafTest System Brochure Download

Complete product info, features and benefits, applications and AOCS vs. SafTest correlation data.