Instruments and Consumables

Testing Types and Kits

Need Your Sample Tested?

Customer Testimonials

Why Choose SafTest™?
Certifications
Reagents meet the rigorous standards established by AOAC and ASTM
Proven
Reagent kits are reproducible, lot-to-lot consistency, scalability, and easy to use
Speed
Evaluate 20 samples per hour, with as little as 10 - 50 μL sample volume
Convenience
Each kit is configured and standardized for analysis of 110 samples at a cost of $4.5 per sample
Safety
Reagents are low hazard and require no toxic chemicals or solvents

SafTest™ Training

AlkalSafe Test Kits are designed to measure alkenal content in oils, tallows, greases, feed, and protein meals in both raw materials and finished products. Alkenals are an indicator of secondary oxidation associated with off odors and off flavors (rancidity).

  • Alkenal Value Overview
  • AlkalSafe Kit
  • Alkenal Analysis
  • AlkalSafe Protocol
Alkenal Analysis with AlkalSafe Kit


What are Alkenals?

Alkenals arise from the degradation of lipid peroxides. Like malonaldehyde, alkenals are products of secondary oxidation. As products of secondary oxidation, the presence of alkenals also is indicative of rancidity. There is evidence that the presence of certain alkenals within the body can affect cellular respiration. The presence of specific alkenals has been linked to such diseases as Alzheimer's. Shown below is one basic structure of an alkenal.


What is AlkalSafe?

The AlkalSafe Kit is designed to provide rapid analysis of the alkenal content of oils, tallows, greases, feed, and protein meals using micro-analytical and membrane separation principles. The AlkalSafe Kit quantitates alkenals through a condensation reaction with a chromagen whose visible spectrum then changes and is read using the SafTest Analyzer. The proprietary AldeSafe Kit can be used for testing solubilized dry and wet food matrices by releasing lipid from the sample matrix using a stabilized reagent (i.e. stabilized isopropanol) and employing mechanical mixing and warming techniques. The solubilized food matrix is then filtered through a membrane and the filtrate is analyzed for alkenals. The significantly shorter sample preparation of 15 to 20 minutes permits data to be obtained in 25 to 30 minutes. MP Biomedicals' AlkalSafe Kit contains all reagents, calibrators, and controls required for testing up to 120 tests. The alkenal value is expressed as µmol/kg or nmol/kg of sample.


Sample Preparation: Semi-solid/solid oils and meals require heating and mechanical disruption
Solid samples require membrane separation
Time Requirement: Sample Preparation 5 to 20 minutes
Analysis time approximately 20 minutes
Limit of Quantitation: 0.0 - 1,200µmol of Alkenal/kg of sample
Interferences: Highly colored samples
Alkenal Value Overview


The AlkalSafe Kit is designed to provide rapid analysis of the alkenal content of oils, tallows, greases, feed, and protein meals using micro-analytical and membrane separation principles. The AlkalSafe Kit quantitates alkenals through a condensation reaction with a chromagen whose visible spectrum then changes and is read using the SafTest Analyzer. The proprietary AldeSafe Kit can be used for testing solubilized dry and wet food matrices by releasing lipid from the sample matrix using a stabilized reagent (i.e. stabilized isopropanol) and employing mechanical mixing and warming techniques. The solubilized food matrix is then filtered through a membrane and the filtrate is analyzed for alkenals. The significantly short sample preparation time of 15 to 20 minutes permits data to be obtained in 25 to 30 minutes. MP Biomedicals' AlkalSafe Kit contains all reagents, calibrators, and controls required for testing up to 120 tests. The alkenal value is expressed as µmol/kg or nmol/kg of sample.

Methodology

To demonstrate method repeatability, accuracy, and linearity, samples of various types of meals and samples of snack products were sent to an independent method validation laboratory for validation. Samples were analyzed using the AOCS Official method AOCS p-Anisidine / Gas Chromatography of Hexanal Content and SafTest's AlkalSafe Kit.


Repeatability and Accuracy Studies

Correlation of AlkalSafe Results and Sensory Results

Correlation of AlkalSafe Results and p-Anisidine for Oil Samples

AlkalSafe - A Better Indicator of Oil Abuse than p-Anisidine

AlkalSafe Protocols



Supporting Technical Information

Browse SafTest's Technical Resource Center for all the technical resources needed to support your decision-making process.



Download the Current SafTest System Brochure Download

Complete product info, features and benefits, applications and AOCS vs. SafTest correlation data.