Please be advised: MP Biomedicals (Ohio) will NOT be shipping product from November 1st through November 9th due to warehouse enhancements, inventory counts, and system changes. We sincerely apologize for any inconvenience this may cause.
The product is tested for purity at a minimum of 20 mg/mL using immunoelectrophoresis. The product is human serum IgA; no trace of IgG, IgM or non-immunoglobulin serum proteins is detected. The total protein is determined using the Biuret procedure, with bovine albumin as the standard. Each vial is overfilled by approximately 10 percent so that a minimum of 5 mg of human serum IgA is obtained upon reconstitution.
Human IgA is purified from pooled human serum using multi-step procedures which may include salt fractionation, gel filtration, ion-exchange chromatography and immunoabsorption. The product is dialyzed into 0.01M sodium phosphate, 0.07M sodium chloride, pH 7.3, filtered through a 0.22 μm filter, vialed and lyophilized.
Purified human serum IgA is suitable for use as a positive control in antibody identification screening techniques and is recommended as an ELISA coating antigen and control.
From human plasma highly purified, immunoabsorbed to remove trace amounts of contaminant proteins
Product is the lyophilized powder of human serum IgA and buffer salts.
Reconstitution: Reconstitute product with 1 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Avoid vigorous shaking of the reconstituted material
Typical Working Concentration
Suggested working dilutions: 1:50 - 1:200 for Tissue Staining; 1:100 - 1:500 for Immunoblotting; 1:400 - 1:2,000 for ELISA
Total Protein: 5.41 mg/mL; Volume: 1 mL
Storage & Handling
Store unopened product at 2-8 °C. Store reconstituted material no longer than a few days at 2-8 °C unless sodium azide is added. For long-term storage, aliquot and freeze at -20 °C
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Hsiao-Hui Yang Recombinant group B streptococcus Beta C protein and a variant with the deletion of its immunoglobulin A-binding site are protective mouse maternal vaccines and effective carriers in conjugate vaccines. Infection & immunity200775, 3455-3461
H. Marie Lacy Essential Role for Neutrophils in Pathogenesis and Adaptive Immunity in Chlamydia caviae Ocular Infections. Infection & immunity201179, 1889-1897