The total protein is measured using the Biuret procedure, with bovine albumin as a standard. Antibody titer is standardized to an in-house standard by equivalence-point precipitation. Each IgG fraction is tested for purity and specificity at a minimum of 40 mg/mL using immunoelectrophoresis. The product is mostly rabbit IgG; no trace of albumin is detected.
The IgG fraction is prepared from the specific rabbit antiserum by delipidation, ammonium sulfate fractionation and anion exchange chromatography. The resulting IgG fraction is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 Ám filter, vialed and lyophilized.
This product is suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. This product is suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers, and for attachment to solid supports for use as an immunoadsorbant. This product is suitable for use as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or tissue immunostaining.
Product is the lyophilized powder of rabbit IgG fraction to human albumin and buffer salts.
Typical Working Concentration
Suggested working dilutions: 1:50 - 1:200 for Tissue Staining; 1:100 - 1:500 for Immunoblotting; 1:400 - 1:2,000 for ELISA/FIA
F. Eun-Hyung Lee Circulating Antibody-Secreting Cells during Acute Respiratory Syncytial Virus Infection in Adults. Journal of Infectious Diseases2010202, 1659-1666
Yutaka Amako Protein Kinase D Negatively Regulates Hepatitis C Virus Secretion through Phosphorylation of Oxysterol-binding Protein and Ceramide Transfer Protein*. Journal of Biological Chemistry2011286, 11265-11274
Shalinee Dhayal Pharmacological characterization of the cytoprotective effects of polyunsaturated fatty acids in insulin-secreting BRIN-BD11 cells. British Journal Pharmacology2011162, 1340-1350
Sreedhar R. Nallapareddy Ligand-Signaled Upregulation of Enterococcus faecalis ace Transcription, a Mechanism for Modulating Host-E. faecalis Interaction. Infection & Immunity200674, 4982-4989
Ratings & Reviews
Ratings & Reviews
No reviews available for this product at this time.
Our team of scientists has experience in all areas of research including Life Science, Chemical Synthesis, Analytical and many others.