0855029 MP BIOMEDICALS
RABBIT IGG FRACTION TO HUMAN ALBUMIN

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Properties

Concentration Total Protein: 15.1 mg/mL; Antibody Protein: 5 mg/mL
Keywords Rabbit Anti human Antibody, Human albumin, Polyclonal antibody
Key Applications Immunoblot
ELISA
Immunohistochemistry
Immunofluorescence
Application Areas Immunoassays
Product Type Antibodies

Description

Analysis Note

The total protein is measured using the Biuret procedure, with bovine albumin as a standard. Antibody titer is standardized to an in-house standard by equivalence-point precipitation. Each IgG fraction is tested for purity and specificity at a minimum of 40 mg/mL using immunoelectrophoresis. The product is mostly rabbit IgG; no trace of albumin is detected.


Preparation Method

The IgG fraction is prepared from the specific rabbit antiserum by delipidation, ammonium sulfate fractionation and anion exchange chromatography. The resulting IgG fraction is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 Ám filter, vialed and lyophilized.


Applications

This product is suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. This product is suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers, and for attachment to solid supports for use as an immunoadsorbant. This product is suitable for use as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or tissue immunostaining.


Product Description

Product is the lyophilized powder of rabbit IgG fraction to human albumin and buffer salts.


Typical Working Concentration

Suggested working dilutions: 1:50 - 1:200 for Tissue Staining; 1:100 - 1:500 for Immunoblotting; 1:400 - 1:2,000 for ELISA/FIA


Trade Name

Cappel™


Price and Availability

SKU-Pack Size Availability Price (USD) Quantity
0855029 - 2 ml In Stock 148.85

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Protocols & References

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