Clear, yellow liquid

Synonyms Folin's phenol reagent, Folin–Denis reagent, FCR

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Preparation Method

A procedure for preparation of the Folin & Ciocalteu's phenol reagent has been published.Dissolve 10 g of sodium tungstate and 2.5 g of sodium molybdate in 70 ml of water. Add 5 ml of 85% phosphoric acid and 10 ml of concentrated hydrochloric acid. Reflux for 10 hours. Add 15 g of lithium sulfate, 5 ml of water, and 1 drop of bromine. Reflux for 15 minutes. Cool to room temperature and bring to 100 ml with water. (Krebs, K.G., et al., in Thin Layer Chromatography, Stahl, E., (ed.), Springer-Verlag, (New York, NY: 1969) p. 878)


The most common usage of Folin & Ciocalteu`S Phenol Reagent reagent is in the Lowry method for determining protein concentration. It can also be used as a spray reagent in chromatographic procedures.

Product Description

Folin & Ciocalteu`S Phenol Reagent is suitable for determination of total protein by Lowry method. It is a mixture of phosphomolybdate and phosphotungstate used for the colorimetric assay of phenolic and polyphenolic antioxidants.


Concentration 2N
Keywords Protein Estimation, Biochemicals and Reagents, Core Bioreagents, Enzyme Substrates, Enzymes, Inhibitors, and Substrates, General Protein Quantitation,Protease Detection Kits, Protein Quantitation, Proteomics, Quantitation Kits and Reagents, Stains and Dyes
Key Applications Protein Estimation and Quantitation
Product Type Proteins, Enzymes & Peptides
Biochemical Category Reagents

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