02154570 MP BIOMEDICALS

FIBROBLAST GROWTH FACTOR, BASIC, HUMAN RECOMBINANT

≥95%, ~0.5 ng/mL (ED50), White Solid

Synonyms FGF2, bFGF, Fibroblast Growth Factor-Basic human

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Properties

Purity ≥95%
Specific Activity ~0.5 ng/mL (ED50)
Suitability Suitable for cell culture
Keywords Angiogenesis, Angiogenic Factors, Cancer Research, Cell Biology, Cell Culture, Cell Signaling and Neuroscience, Cytokines and Growth Factors, Cytokines, Growth Factors and Chemokines for Stem Cell Differentiation, Growth Factors and Hormones, Chemokines for Stem Cell Biology, Cytokines, Growth Factors, and Chemokines for Stem Cell Expansion, Embryonic Stem Cell Biology Growth Factors, FGF, Fibroblast Growth Factors (FGF), General Stem Cell Biology, Growth Factors and Cytokines, Hematopoietic Stem Cell Biology, Hematopoietic Stem Cell Cytokine and Growth Factors, Life Science Reagents for Cell Culture, Mesenchymal Stem Cell Biology, Mesenchymal Stem Cell Biology Growth Factors, Nerve Growth Factors and Cytokines, Neural Stem Cell Biology, Reagents and Supplements, Stem Cell Biology, Stem Cell Differentiation, Stem Cell Expansion, Stem Cell Reprogramming
Key Applications Maintenance of human embryonic stem cells

Description

Applications

Fibroblast Growth Factor, Basic, Human Recombinant is required for the maintenance of human embryonic stem cells in culture, and various other stem cell lines such as mesenchymal stem (stroma) cells (MSC).


Product Description

Fibroblast Growth Factor-basic (FGF-b or FGF-2) is a heparin binding growth factor which stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal and endothelial cells. FGF-b also exerts a potent angiogenic activity in vivo. Human FGF-b is a 17.2 kDa protein containing 155 amino acid residues.


Biochem/physiol Actions

Fibroblast growth factor-basic (FGF-Basic) is found in basement membranes and sub-endothelial extracellular matrix. FGF-Basic is induced early in the development of the embryo where it is necessary (but not sufficient) for pluripotency and self-renewal of embryonic stem cells (ESC) and lineage defined stem cells; wherein it functions to support proliferation and inhibit differentiation. Self-renewal of hESC depends upon activation of the Activin/Nodal/Smad2,3 branch and suppression of the BMP/GDF/Smad5 branch of the TGFβ-family cell signaling program. FGF-Basic is a competence factor for Nodal support of pluripotency. Activin-A is necessary and sufficient to maintain stemness. It induces the expression of both Nodal and FGF-Basic in human ES cells.


Recommended Use

Reconstitution: FGF-b is sensitive to acidic conditions. For best stability, the lyophilized FGF-b should be reconstituted in 5 mM Tris pH 7.6 to a concentration of 1 mg/mL. This solution can be diluted into other buffered solutions.


Typical Working Concentration

For most in vitro applications, FGF-b exerts its biological activity in the concentration range of 0.1 to 10.0 ng/mL.


Price and Availability

SKU-Pack Size Availability Price (USD) Quantity
0215457050 - 50 μg Call for Price -
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