02154133 MP BIOMEDICALS

TRANSFORMING GROWTH FACTOR-β1, PORCINE

from Porcine Platelets

≥97%, Activity: ED50: 0.02 - 0.06 ng/mL, White Solid

Synonyms TGF- 1, Transforming Growth Factor-β1 from porcine platelets

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Properties

Purity ≥97%
Specific Activity Activity: ED50: 0.02 - 0.06 ng/mL
Suitability Suitable for cell culture
Keywords Cancer Research, Cell Biology, Cell Culture, Cell Signaling and Neuroscience, Cytokines & Growth Factors, Cytokines and Growth Factors, Cytokines, Growth Factors and Chemokines for Stem Cell Differentiation, Cytokines, Growth Factors and Hormones, Cytokines, Growth Factors, Chemokines for Stem Cell Biology, Cytokines, Growth Factors, and Chemokines for Stem Cell Expansion, Embryonic Stem Cell Biology Growth Factors, General Stem Cell Biology, Growth Factors, Growth Factors and Cytokines, Hematopoietic Stem Cell Biology, Hematopoietic Stem Cell Cytokine and Growth Factors, Mesenchymal Stem Cell Biology, Mesenchymal Stem Cell Biology Growth Factors, Reagents and Supplements, Stem Cell Biology, Stem Cell Differentiation, Stem Cell Expansion, Stem Cell Reprogramming, TGF-β, Transforming Growth Factor-β (TGF-β) and Superfamily, Transforming Growth Factor-b Superfamily Proteins and Peptides, Transforming Growth Factors
Key Applications Multifunctional protein capable of influencing cell proliferation, differentiation, and a variety of cellular functions.

Description

Analysis Note

Unit Definition: measured by the dose dependent inhibition of 3H-thymidine uptake by murine IL-4 dependent HT-2 cell line.


Applications

Transforming Growth Factor-β1 is a multifunctional protein capable of influencing cell proliferation, differentiation, and a variety of cellular functions. TGF-β stimulates the growth of cells of mesenchymal origin, but inhibits growth of hepatocytes, T and B lymphocytes and epithelial cells. TGF-β is known to interact with several agents including EGF, FGF, TGF-α, PDGF, and IL-2. TGF-β is an important mediator of the formation of the extracellular matrix, generally with activities that stimulate the formation of the extracellular matrix and inhibit the degradation ofthe extracellular matrix. TGF-β is a potent inhibitor of the myogenesis of both skeletal and cardiac muscle cells in vitro. Porcine TGF-β1 is identical in amino acid composition to human TGF-β1.


Product Description

Transforming Growth Factor beta (TGF-β) is a stable, multifunctional polypeptide growth factor. While specific receptors for this protein have been found on almost all mammalian cell types thus far examined, the effect of the molecule varies depending on the cell type and growth conditions. Generally, TGF-β is stimulatory for cells of mesenchymal origin and inhibitory for cells of epithelial or neuroectodernal origin. It is know known that the originally described form of TGF-β, now described as TGF-β1, is only one of a family of regulatory proteins consisting of a number of proteins distantly related to TGF-β1 (30 - 40% sequence homology) and a number of more closely related proteins (70-80% sequence homology) designated TGF-β2, TGF-β1.2, TGF-β3, TGF-β4, and TGF-β5. TGF-β1 has been found in the highest concentration in human platelets and mammalian bone, but is produced by many cell types in smaller amounts. TGF-β2 has been found in the highest concentration in porcine platelets and mammalian bone, but again is also produced by many types of cells. The heterodimer, TGF-β1.2, has so far been found only in small amounts in porcine platelets. TGF-β3 has been detected in human, porcine, and avian sources, mainly in sells of mesenchymal origin, suggesting a distribution in other types of cells has not yet been characterized. TGF-β5 has been detected only in Zenopus embryos. TGF-β1, TGF-β2, and TGF-β1.2 appear to be largely equivalent in biological activity, although there appear to be differences in binding to certain types of receptors, and there are a few reports of differential responses to TGF-β1 and TGF-β2. The other forms of TGF-β have not been obtained in large quantities from natural sources and recombinant expression of these proteins is only now providing sufficient amounts of protein for studies on the comparative biological activities of these forms of TGF-β to be carried out.


Biochem/physiol Actions

Transforming growth factor-β1 (TGF-β1) is produced by many cell types, but is reported to be most concentrated in mammalian platelets, where it is present at approximately four times the level of TGF-β2.


Unit Definition

Measured by the dose dependent inhibition of 3H-thymidine uptake by murine IL-4 dependent HT-2 cell line.


Recommended Use

Reconstiution: It is recommended that sterile 4 mM HCI containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 1 μg/mL.


Price and Availability

SKU-Pack Size Availability Price (USD) Quantity
0215413301 - 1 μg In Stock 789.60

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