02153322 MP BIOMEDICALS
PHASEOLUS VULGARIS AGGLUTININ from Red Kidney Bean

White Powder

Synonyms PHA-P, Lectin from Phaseolus vulgaris, PHA

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Properties

Keywords Phaseolus vulgaris (Red kidney bean), Cell Biology, Cell Signaling and Neuroscience, Glycan Recognizing Proteins, Glycobiology, Immune Cell Signaling and Blood, Immune System Regulation, L, Lectins, Lectins and Lectin Antibodies, Post-Translational Modification, Proteomics
Key Applications Iontophoresis
Blood grouping and erythrocyte polyagglutination studies
Mitogenic stimulation of lymphocytes
Product Type Proteins, Enzymes & Peptides
Presentation White Powder
Format Powder

Description

Applications

Phaseolus vulgaris Agglutinin is used in wide variety of applications in vitro, including blood grouping and erythrocyte polyagglutination studies; Mitogenic stimulation of lymphocytes; Lymphocyte subpopulation studies; Fractionation of cells and other particles; Histochemical studies of normal and pathological conditions.


Product Description

Lectins are proteins or glycoproteins of non-immune origin that agglutinate cells and/or precipitate complex carbohydrates. Lectins are capable of binding glycoproteins even in presence of various detergents.


Biochem/physiol Actions

PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity. PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.


Recommended Use

Reconstitute with 5 mL distilled water for a final protein concentration of 1mg/mL in 0.40 mM sodium phosphate -6 mM NaCI, pH 7.45. Reconstitute with 0.2 mL distilled water for a final protein concentration of 25mg/mL (2.5%) in Buffer. This concentration of the lectin has been used for iontophoresis and neuron transport.


Price and Availability

SKU-Pack Size Availability Price (USD) Quantity
0215332201 - 1 mg 3-4 weeks from Order 46.80
0215332205 - 5 mg 3-4 weeks from Order 144.90

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