02152284 MP BIOMEDICALS

β-GLUCURONIDASE

from Helix pomatia

β-Glucuronidase Activity: >50,000 units per mL,Sulfatase Activity: ≤5000 u/mL, Brown Solution

Synonyms β-D-Glucuronide glucuronosohydrolase

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Description

Applications

β-Glucuronidases are routinely used for the enzymatic hydrolysis of glucuronides from Serum plasma and other fluids prior to analysis by enzyme immunoassay, mass spectrometry, gas chromatography, high performance liquid chromatography, or other means.β-glucuronidase is used as a reporter gene in GUS assays to monitor gene expression.


Product Description

Beta-glucuronidases are members of the glycosidase family of enzymes that catalyze breakdown of complex carbohydrates. Human β-glucuronidase is a type of glucuronidase that catalyzes hydrolysis of β-D-glucuronic acid residues from the non-reducing end of mucopolysaccharides such as heparan sulfate. In human gut β-glucuronidase converts conjugated bilirubin to the unconjugated form for reabsorption.


Biochem/physiol Actions

β-glucuronidase catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption.β-Glucuronidase from Helix pomatia is a mixture of enzymes. Inhibitors: D-glucuronic acid, D-galacturonic acid, D-glucaro-1, 4-lactone


Unit Definition

β-Glucuronidase Unit Definition : One unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hour at pH 5.0 and 37 °C. Sulfatase Unit Definition : One unit will hydrolyze 1.0 μmole of p-nitrocatechol sulfate per hour at pH 5.0 and 37 °C.


Properties

Specific Activity β-Glucuronidase Activity: >50,000 units per mL,Sulfatase Activity: ≤5000 u/mL
Keywords Carbohydrate hydrolysis & PTM analysis, Biochemicals , Carbohydrate Hydrolysis, Core Bioreagents, Diagnostic and Analytical Enzymes, Enzymes, Inhibitors, and Substrates, Molluskan Source
Key Applications Used as reporter gene
Enzymatic hydrolysis of glucuronides
Application Areas Molecular Biology,Diagnostic and Analytical Enzyme
Product Type Proteins, Enzymes & Peptides

Price and Availability

SKU-Pack Size Availability Price (USD) Quantity
0215228401 - 1 mL Call for Price -
0215228402 - 2 mL Call for Price -
0215228405 - 5 mL Call for Price -
0215228410 - 10 mL Call for Price -
0215228450 - 50 mL Call for Price -
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Not less than 1000 U/mg protein (approximately 500 U/mg material), Helix pomatia, brown freeze-dried powder

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