Isolate Influenza Virus Particles Using FastPrep-24:
The department of Virology of the Erasmus Medical Center in Rotterdam, Netherlands, has successfully optimized the isolation of Influenza A / H3N2 viruses from murine lung tissues by using the FastPrep-24 instrument. These astonishing results were published in the peer-reviewed PLoS ONE publication; Bodewes R, Kreijtz JHCM, Baas C, Geelhoed-Mieras MM, de Mutsert G, et al. 2009, Vaccination Against Human Influenza A/H3N2 Virus Prevents the Induction of Heterosubtypic Immunity Against Lethal Infection with Avian Influenza A/H5N1 Virus. PLoS ONE 4(5): e5538 . doi:10.1371/journal.pone.0005538.
MP Biomedicals has released a condensed protocol for whole viral particles isolation using the FastPrep-24 system and lysing matrices. Click the link to download a copy of this protocol. For further questions or information regarding the FastPrep-24 or this protocol, please contact one of our molecular biology experts by email: [email protected]