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Culture Media & Reagents

Culture Media Encapsulated Media
MP Biomedicals manufactures and supplies an excellent selection of E.coli growth media for almost any molecular biology application. Specialty, high yield formulations, tried -and-true LB Agar, and even basic ingredients of growth media are available in multiple formats. Each recipe is subjected to extensive quantitative testing an dis Molecular Biology Certified™ through qualitative tests such as cell density and plasmid yield. For ultimate yield and convenience, our patented media capsules eliminate the waste, inaccuracies, and mess associated with weighing out bulk powder. Pre-mixed powders are also available in single-use or in larger bottles and drums. We also offer liquid media, pre-poured plates and custom packaging options.
Available in Bottles or NEW Blister packaging!
Encapsulated LB Media not only provides the ultimate in ease and convenience, but also comes in an easy-to-use blister package.

We've taken our most popular growth media and put it into an easy-to-handle blister package, thus eliminating possible contamination, allowing for easier storage and increased freshness.
Yeast Zymolyase
MP Biomedicals is a leading provider of high quality pre-mixed media formulations for culturing yeasts - S. cerevisiare and S.pombe. Our wide range of yeast nitrogen bases, sugars and amino acid dropout mixtures can be mixed together to form almost any complete media. General media formulations include YPD/YEPD, YEP, YE and YES mixtures. Both synthetic and minimal defined media formulations are also available. We offer these media in convenient and custom formulations.

Synthetic Defined Yeast Media includes all necessary components for selective growth of Saccharomyces cerevisiae. MP Biomedicals offers Synthetic Defined broth (SD) and Synthetic Defined Agar (SDA) in a variety of formats for flexibility and convenience.Synthetic Defined Media (SD) can be ordered in a number of ways:

A complete SD or SDA A Drop Out Base (DOB) or Drop Out Base with Agar (DOBA) plus the appropriate amino acid supplement mixture (CSM) A Yeast Nitrogen Base (YNB), a carbon source, the appropriate amino acid supplement mixture(CSM), and agar if desired All raw materials separately.
Synthetic Defined Yeast Media
DOB Medium
26.7 g/L
Contents L:1.7g YNB,5G Ammonium Sulfate 20g Dextrose
DOBA Medium
43.7 g/L
Contents L:DOB,17g Agar-Y
Zymolyase 20T and 100T are used to lyse living yeast cell walls to produce protoplast or spheroplast of various strains of yeast cells. It may also be used for glucan hydrolysis. The primary enzyme in this preparation responsible for lysis of viable yeast cells in -1,3-glucan laminaripentaohydrolase. It hydrolyzes glucose polymers at the -1,3-glucan linkages releasing laminaripentaose as the principal product.

Zymolyase is an enzyme preparation from a submerged culture of Arthrobacter luteus which effectively lyses cell walls of viable yeast cells. This enzyme is offered in two different concentrations:
  • 20T - This preparation is a lyophilized powder prepared by salting out from the culture fluid with ammonium sulfate and contains 20,000 units enzyme/g.
  • 100T - This preparation is lyophilized enzyme partially purified by affinity chromatography and contains 100,000 units enzyme/g.
Catalog Number Name Pack Size
08320931 ZYMOLYASE 100T 500 mg
08320932 ZYMOLYASE 100T 250 mg
08320921 ZYMOLYASE 20T 1 g

MP Biomedicals offers the highest quality and a broad range in mammalian cell culture media, serum free media, supplements and antibiotics. Our products have been highly validated and cited in scientific publications which provides you the confidence in your research results.

Best in quality
Validation based on refereed publications
Unique and wide range portfolio
Convenient packaging for various needs
Custom formulations made to your specifications
Featured cell Culture Reagents:
Mammalian Cell Culture Media and Salt Solutions

Classical media in powdered and liquid formats for mammalian cell culture applications

The cell culture media products are designed to support optimal cell growth under defined conditions. These products offer the best performance for your cells and are guaranteed to be of the highest quality and consistency.

Our media guarantees your satisfaction:
All products undergo rigorous quality control testing and are tested for endotoxin and toxicity.
Salt solutions are produced without sodium bicarbonate to increase stability.
Catalog Number Name Pack Size
91260349 RPMI 1640 Medium 100 ml
91260354 RPMI 1640 Medium 500 ml
91260454 RPMI 1640 Medium 500 ml
91629754 RPMI 1640 Medium 500 ml
91629149 RPMI 1640 Medium 100 ml
91260254 RPMI 1640 Medium 500 ml
91646454 RPMI 1640 Medium 500 ml
91646854 RPMI 1640 Medium 500 ml
91060120 RPMI 1640 Medium 10x1 L
91060122 RPMI 1640 Medium 1x10 L
91060124 RPMI 1640 Medium 1x50 L
91233254 DULBECCO'S MODIFICATION OF EAGLE'S MEDIUM 500 ml
91233354 DULBECCO'S MODIFICATION OF EAGLE'S MEDIUM 500 ml
91642149 DULBECCO'S MODIFICATION OF EAGLE'S MEDIUM 100 ml
91642254 DULBECCO'S MODIFICATION OF EAGLE'S MEDIUM 500 ml
91642354 DULBECCO'S MODIFICATION OF EAGLE'S MEDIUM 500 ml
91642449 DULBECCO'S MODIFICATION OF EAGLE'S MEDIUM 100 ml
91642454 DULBECCO'S MODIFICATION OF EAGLE'S MEDIUM 500 ml
91642954 DULBECCO'S MODIFICATION OF EAGLE'S MEDIUM 500 ml
91033120 DULBECCO'S MODIFICATION OF EAGLE'S MEDIUM 10 x 1 L
91033122 DULBECCO'S MODIFICATION OF EAGLE'S MEDIUM 1x10 L
91240449 Ham's F-10 Nutrient Mixture 100 ml
91440049 Ham's F-10 MEDIUM 100 ml
91040122 Ham's F-10 Nutrient Mixture 1x10 L
91242354 Ham's F-12 NUTRIENT MIXTURE 500 ml
91042122 Ham's F-12 NUTRIENT MIXTURE 1 x 10 L
91047122 BioRich 1 1x10 L
91046822 Dulbecco's Modification of Eagle's Medium/Ham'S F-12 Medium 1 x 10 L
91032322 JOKLIK'S MODIFICATION OF MINIMUM ESSENTIAL MEDIUM EAGLE 1x10 L
91210354 MINIMUM ESSENTIAL MEDIUM EAGLE 500 ml
91210254 MINIMUM ESSENTIAL MEDIUM EAGLE 500 ml
091210254X6 MINIMUM ESSENTIAL MEDIUM EAGLE 6 x 500 ml
91622049 MINIMUM ESSENTIAL MEDIUM EAGLE 100 ml
91641454 MINIMUM ESSENTIAL MEDIUM EAGLE 500 ml
91213454 MINIMUM ESSENTIAL MEDIUM EAGLE 500 ml
91213254 MINIMUM ESSENTIAL MEDIUM EAGLE 500 ml
91410054 MINIMUM ESSENTIAL MEDIUM EAGLE 500 ml
91601149 Minimum Essential Medium Eagle Amino Acids 100 ml
91010120 MINIMUM ESSENTIAL MEDIUM EAGLE 10x1 L
91031120 MINIMUM ESSENTIAL MEDIUM EAGLE 10 x 1 L
91600449 Basal Medium Eagle Vitamin Concentrate 100 ml
Primary Culture & Leucocytes Media
1X McCoy 5A Medium (Park and Terasaki Modification) without L-glutamine This medium supports the growth of primary cultures derived from normal bone marrow, testes, mouse kidney, skin, gingiva, rat embryo and other tissues. MP's media are also suited for the propagation of leukocytes, biopsy tissues and the most demanding primary and continuous cell types.
Medium 199 with Earl's Salts (E199)
Medium 199 can be used with a broad range of species including the culturing of non-transformed cell types. It may be used for vaccine production and the in vitro cultivation of rat lens tissues and primary mouse pancreatic epithelial explants. It is available in either Earle's or Hank's salts.
Catalog Number Name Pack Size
91255354 MCCOY'S 5A MEDIUM 500 ml
91692149 MCCOY'S 5A MEDIUM 100 ml
91220449 MEDIUM 199 100 ml
91020120 MEDIUM 199 10x1 L
91023120 MEDIUM 199 10x1 L
Serum Free Media and Supplements
The use of fetal bovine serum in cell culture media can lead to concerns arising from biological contamination, higher complexity in assays and downstream purification. Additionally, lot-to-lot variation in performance impacts sourcing and testing. MP Biomedicals wide range of Serum Free products puts your mind at ease when it comes to cell culture. Our serum free products are:
Well defined with minimal variability
Better control and consistent performance
Ideal for evaluation of cellular functions in assays
Preserve your serum free cell line - Cellvation™
Cellvation™ is a cryopreservation medium which contains neither DMSO nor Serum and is ideally suited for serum-free cultures. Cellvation™ offers
Optimal recovery and faster cell attachment
Suitability for all types of serum-dependent cell lines
Solution which is ready to use
MP-Hybridoma® Solutions
Focused on hybridoma research? Look no further than MP Biomedicals for all of your needs pertaining to serum free media and supplements. Our Serum free medium offers you distinct advantages:
MP-Hybridoma® Medium, Serum Free (NEW)
Low protein content (11ug/ml)
Chemically defined, no animal derived products
Supports hybridoma cells, as well as lymphoid and epithelial cells
Opti Clone™ II Hybridoma Cloning Factor
Opti-Clone™ II is a serum-free growth medium supplement which improves the cloning efficiency of murine B cell hybridomas.
Highly efficient growth supplement - up to 25% higher yields of mAbs
Increases cell health - higher viability of cells
Protein free - facilitates downstream purification
Chemically defined - no variation in performance means consistent results
ChromatoPur™ Proteins (NEW)
ChromatoPur™ proteins have been purified by chromatography and ensure the highest purity, consistency and activity in cell culture. Unlike with other extraction procedures (e.g. Cohn, Heat Shock) ChromatoPur™ proteins are more biologically intact and highly retain their cell growth stimulating activity. Proteins are purified from New Zealand sourced material using cGMP in an ISO 9001 certified facility which is audited by NZFSA-MAF.
ChromatoPur™ proteins offers:
Superior performance over Cohn's Albumin Fraction V
Intact proteins with high activity
Highest purity and consistency
Media Supplements
Supplemental nutrients and reagents are essential for many cell cultures. Additives such as antibiotics and buffers are frequently used to prevent bacterial contamination, control pH and monitor culture conditions. Amino acids and vitamins are useful for enriched media.
MP Biomedicals offers a wide choice of media supplements to meet your cell culture needs (see our web site for complete listing of media supplements).
Antibiotic Solutions
Antibiotic solutions from MP Biomedicals are proven effective at keeping mammalian cell cultures free of bacterial or fungal contaminations. Antibiotic solutions are also used for clonal selection.
Along with our Plant & Insect nucleic acid purification kits we are pleased to offer an accompanying line of cell culture media. Our plant cell culture medias come in the standard Murashige & Skoog, Gamborg's, and Chu's formulations. Additionally, we also offer specialized plant cell culture media and tools.

For years, insect cell culture has been an important tool in molecular biology research, particularly for those involved in Drosophila research. Our line of CELLect insect cell culture media offers the insect biologist a range of options for their samples.