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Immuno Solutions - Detection Reagents
Alkaline Phosphatse (AP) and Horseadish Peroxidase (HRP) are two major enzymes that are used for secondary detection. MP Biomedicals has a broad range of anti-enzyme products available in various conjugation forms from many differentspecies
Streptavidin, avidin and biotin conjugated with AP, HRP or FITC are ideal to amplify signals in immunochemistry, ELISA, flow cytometry and Western botting. Our AP Avidin, Avidin-HRP and Streptavidin-FITC for use as secondary reagents allow further enhancement of signal.
Protein A and G
Recombinant protein A (from Streptococcus sp) binds immunoglobulins (primarily IgG) at the Fc region with high affinity. This unique property provides a rapid, simple and economical method for purification and analysis of a broad- spectrum of antibodies. AP Biotin or colloidal gold conjugated Protein A are versatile reagents which can be used for many immunological assays. Recombinant protein G is conjugated with aminohexanoyl biotin N-hydroxysuccinimide under optimum conditions and is free of unconjugated biotin. This conjugate reacts with the Fc portions of immunoglobulins of various species.