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Immuno Solutions - Detect
MP Immunodetection reagents encompass 10,000+ mono, polyclonal and secondary antibodies, purified antigens, detection reagents and supplementary reagents. These reagents are the best in quality, highly validated, cited in refereed journals and offer the best value. Try our best sellers!
Antibodies for Detection of GFP - best in quality, reliable and most recognizable!
Clone 3E6 is an IgG2a monoclonal antibody that recognizes the native forms of Green Fluorescent Protein (GFP), GFP variants and GFP-fusion proteins. Clone 3E6 is highly specific and sensitive for applications in mammalian and bacterial cells;
Immunoprecipitation: 1-5 mg of antibody/sample
Immunocytochemistry: 1:100-1:500 dilution
ELISA: 1:1000-1:5000 dilution
Coating antibody: 2-10 mg/ml
Purification of GFP-fusion using GFP antibody coupled to sepharose beads
Determine optimal dilution based on your application
Embryo stained with anti-GFP monoclonal antibody clone 3E6 from MP Biomedicals
Detect your GFP fusion protein in Western Blots!
Clone 11E5 is a mouse IgG1 monoclonal antibody that recognizes the denatured form of the GFP, GFP variants and GFP - fusion proteins. Detect as little as 10ng of GFP using colorimetric methods or pictogram quantities with chemiluminescent techniques.
Western blot analysis and immunoprecipitation of the GFP-tagged proteins using MP Biomedicals antibody against GFP.
Monoclonal Antibodies to Detect Actin and Tubulin
Antibodies to cytoskeletal proteins are widely used for protein loading controls or specific studies such as apoptosis. MP Antibodies to actin (clone c4) have demonstrated specificity directed towards all six known vertebrate isoactins. Our anti-tubulin monoclonal antibodies enable researchers to visualize mictotubules in fixed cells and in fixed or frozen tissue sections from various species.
Detects all know actins or tubulins
Excellent positive control for Western Blots, Immunofluorescence and Immunohistochemistry
No known spurious activities
Actin intracellular visualisation using MP Biomedicals antibody against Actin
Primary Antibodies - Epitope Tag Antibodies
Epitope Tags are small peptides easily identified by specific antibodies. The fusion of these few amino acids to the targeted protein facilitates purification and detection procedures. MP Biomedicals offers three epitope tagged, high quality antibodies with outstanding specificity and efficient performance in various applications:
Anti-c-Myc is able to detect the fusion protein of the Myc epitope cloned either to the amino- or carboxy- termini of targeted proteins. This antibody is suitable for ELISA and Western blotting and was tested against both the immunizing peptide and Myc-tagged recombinant proteins.
Antibodies to 6xHIS epitope tags H-H-H-H-H-H (also called anti-His, anti-hexahistidine, anti-polyhistidine, etc) can recognize antigenic determinants (epitopes) on recombinant proteins. The antibody recognizes the His-tag fused to amino- or carboxy-termini of targeted proteins in transfected or transformed cells.
The FLAG epitope tag DYKDDDDK (N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C) is a small but highly immunogenic peptide, which allows fusion proteins to retain their original conformation and function. The hydrophilic character of FLAG peptide increases the likelihood that it will be located on the surface of the fusion protein where it is accessible to antibodies.
High Throughput Monoclonal Antibodies
MP Biomedicals offers you 5000+ monoclonal antibodies to cellular targets which are internal and external. The antibodies are highly purified, application tested and guaranteed for quality. Our monoclonal antibodies offer you the best performance for value:
Immunogens based on gene sequence
Immunogens expressed using recombinant DNA technology
Combination of low dose immunization techniques and use of a proprietary adjuvant
Antibodies are purified using Protein A Chromatography
Immunogens mimic the native proteins
Use an in vitro wheat germ system to express the immunogens
Expressed peptide mirrors the confirmation and folding of the native protein
Antibodies are tested in appropriate cell lines and tissues
Each antibody is extensively application tested
Dilutions recommended for each application
Secondary Antibodies - First Class Quality and Service
MP Cappel secondary antibodies offers the highest specificity, quality, titer and the widest selection against human, mouse and rabbit immunoglobulins.
Multiple forms are available: unconjugated, enzyme or fluoro conjugated.
Enzyme (Alkaline phosphatase (AP) or Horseradish peroxidase (HRP) conjugated antibodies are suitable for EIA, ELISA, Blot Immunostaining and cell/tissue staining.
Fluorochrome conjugated antibodies are used for immunofluorescence assays, cell/tissue staining, and fluorescence-activated cell sorting (FACS).
Antibodies to Rabbit Immunoglobulins