The MP Diagnostics HTLV-I/II ELISA 4.0 System is an enzyme-linked immunosorbent assay intended for the detection of antibodies to Human T-cell Lymphotropic Virus type I and type II in human serum or plasma.
This kit for available as Research Use Only (RUO) in the United States. Please contact your regional sales team for the most recent availability in your region.
Chemical & Biological Principles of the Procedure
The wells of the polystyrene microplate strips are coated with a mixture of three different HTLV recombinant proteins, which correspond to the highly antigenic segments of HTLV-I and HTLV-II viruses.
The conjugate is based on a tri-fusion recombinant protein, which is labeled with horseradish peroxidase (HRP). The tri-fusion antigen is generated by cloning of three cDNA fragments coding for the three HTLV recombinant proteins into a single vector.
Human sera or plasma, diluted in the diluent containing the conjugate, are incubated in these coated wells. HTLV-I/II specific antibodies (IgA, IgG and IgM), if present, will bind to both the antigens immobilised on the solid phase and the tri-fusion antigen of the conjugate.
After incubation, the wells are thoroughly washed to remove unbound materials. A colorless substrate solution containing chromogen 3,3',5,5' - tetramethylbenzidine (TMB) is then added to each well.
The presence of specific antibodies is indicated by the presence of a blue color after incubation, which changes to yellow when the color reaction is terminated by the addition of sulphuric acid.
The intensity of the resulting yellow product is measured at 450nm using a spectrophotometer and is proportional to the amount of antibodies present in the specimen.
The health information contained herein is provided for educational, reference purposes only, and is not intended to substitute professional medical advice, diagnosis, or treatment with a healthcare provider. All decisions regarding patient care must be made with a healthcare provider, considering the unique characteristics of the patient. Please check the availability and relevance of the information in your respective country.