Contact Us

(800) 854-0530

Hours of Operation:

8:00 AM - 6:00 PM EST

Events Calendar

Dec 07

24th Meeting of the French Society of Toxinology (SFET)

Dec 07, 2017

Institut Pasteur, Paris, FRANCE

Dec 14

Molecular Microbial Ecology Group Meeting (MMEG 2017)

Dec 14, 2017

University of Warwick. Coventry, UK

Recent Zika virus clinical cases have emphasized the importance of research characterize arboviruses and specifically related  members of the genus Flavivirus that are responsible for related diseases including yellow fever, dengue, West Nile virus, and various encephalitises and hemorrhagic fevers.  The FastPrep Suite of Sample Preparation instruments, Lysing Matrix Tubes and Purification Kits provides researchers with quantitative sample preparation and extraction and isolation of nucleic acids, proteins and small molecules from virtually any sample type including insects, infected tissue, soil, water, bodily fluids and feces.  Resulting pure products are ready for immediate downstream analysis for understanding the mechanisms of virus entry, replication, and the antiviral immune response, as well as developing reliable detection methods, finding effective treatments, and controlling the spread of disease.

 


Attainable goals after FastPrep® processing achieving high concentration and yield.

  • Detection of viral RNA in virtually any sample by using reverse transcription PCR (RT-PCR) to determine infection. 2,5
  • DNA sequence analysis to characterize symbiont to vector/host relationships for transmission fitness and for vector control strategies 1,4
  • Nucleic acids and protein analysis to identify/confirm vector taxonomy and phylogeny, and to determine likelihood estimates of vector population infections (MLEs) 1,3,6

 


Isolation Workflow

  Lysing Matrix A
Lysing Matrix B
Lysing Matrix C
Lysing Matrix D
Lysing Matrix E
FastPrep 24 5G
FastPrep 96
SuperFastPrep-2
FastRNA Kits
FastDNA Kits
 

  FastRNA® Pro Green Kit

Figure 1:
Mouse total RNA extracted with the FastRNA® Pro Green Kit. 

Approximately 2% of the total RNA isolated from 100 mg frozen tissue was loaded on to a 1.2% denaturing agarose gel (1XMOPS). 

Lane 1: tail; Lane 2: kidney; Lane 3: liver; Lane 4: ear; Lane 5: brain; Lane 6: 0.24-9.5kb RNA Ladder.

 


Publications 

    1. Katz, Marly B, Utilizing Molecular Techniques to Distinguish Mosquito Species and Populations, 2014, Biotechniques
    2. Kim H, et al., Detection of Japanese Encephalitis Virus Genotype V in Culex orientalis and Culex pipiens (Diptera: Culicidae) in Korea, 2015, PLoS ONE 10(2), e0116547
    3. Dieme, C, et al., Accurate identification of Culicidae at aquatic developmental stages by MALDI-TOF MS profiling, 2014, Parasites & Vectors, 7(544),
    4. Ren, X, et al., Anopheles gambiae densovirus (AgDNV) has negligible effects on adult survival and transcriptome of its mosquito host, 2014, PeerJ 2, e584, DOI 10.7717_peerj.584
    5. Borovsky, D, et al., Synergy Between Aedes aegypti Trypsin Modulating Oostatic Factor and δ-Endotoxins, 2010, The Open Toxinology Journal, 3, 141-150
    6. Johnson, PDR, et al., Mycobacterium ulcerans in Mosquitoes Captured during Outbreak of Buruli Ulcer, Southeastern Australia, 2007, Emerging Infectious Diseases, 13 (11)