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Isolation of RNA in the Zika Virus
Recent Zika virus clinical cases have emphasized the importance of research characterize arboviruses and specifically related members of the genus Flavivirus that are responsible for related diseases including yellow fever, dengue, West Nile virus, and various encephalitises and hemorrhagic fevers. The FastPrep Suite of Sample Preparation instruments, Lysing Matrix Tubes and Purification Kits provides researchers with quantitative sample preparation and extraction and isolation of nucleic acids, proteins and small molecules from virtually any sample type including insects, infected tissue, soil, water, bodily fluids and feces. Resulting pure products are ready for immediate downstream analysis for understanding the mechanisms of virus entry, replication, and the antiviral immune response, as well as developing reliable detection methods, finding effective treatments, and controlling the spread of disease.
Attainable goals after FastPrep® processing achieving high concentration and yield.
FastRNA® Pro Green Kit
Approximately 2% of the total RNA isolated from 100 mg frozen tissue was loaded on to a 1.2% denaturing agarose gel (1XMOPS).
Lane 1: tail; Lane 2: kidney; Lane 3: liver; Lane 4: ear; Lane 5: brain; Lane 6: 0.24-9.5kb RNA Ladder.