Contact Us

800 854 0530

Hours of Operation:

8:00 AM - 7:00 PM EST

Events Calendar

Dec 14

Molecular Microbial Ecology Group Meeting (MMEG 2017)

Dec 14, 2017

University of Warwick. Coventry, UK

MP Biomedicals provides a complete solution for safe cell culture and reliable results!

Working with mammalian cell lines requires mycoplasma-free conditions, but oftentimes cell cultures get contaminated from multiple sources which can become a nuisance. The presence of mycoplasma can literally alter many cellular processes and cause malfunctions of cells in proliferation, protein synthesis, DNA transfection etc. Early detection and fast elimination can ensure the reliability, reproducibility, and consistency of your cell culture research.

The most convenient and effective option for eliminating mycoplasma infection in cell cultures is to add mycoplasma removal agent (MRA) to remove and prevent many types of mycoplasmas with reliable results. MRA ensures high elimination efficiency and optimal cell viability at the lowest dose for the shortest treatment time (Fig. 1). Only 0.5 µg/mL for one week can keep cell culture mycoplasma free (Fig. 2), as referenced in 550+ leading publications

 


Fig 1. Mycoplasma removal efficiency comparison among competitors in concentration and average treatment time (Reference No. 13). *indicates 5~10% v/v concentration.

 


Fig 2. Illustration of mycoplasma removal in cell culture before and after MRA treatment for one week.

Mycoplasma Removal Agent ensures:

  • Fastest (1 week) with lowest dosage (0.5µg/ml) for total mycoplasma elimination
  • Elimination of multiple mycoplasma species
  • Prevention of recontamination
  • Sterility and optimal cell viability
  • Compatible with mammalian cell lines
  • Extensive citations in publications (over 550)

 

Mycoplasma Stain Kit:

  • Hoechst 33258 staining, Tissue Culture Association procedure # 75361 for reliability
  • Detection of mycoplasma and bacteria, yeast, fungi for versatility
  • Test performed in less than 2 hours for efficiency and speed
  • Stain, diluent and mounting medium included in the kit for a complete solution

 

Selected publications using Mycoplasma Removal Agent (MRA) from MP Biomedicals:

  1. Kee J-Y, Han Y-H, Kim D-S, Mun J-G, Park S-H, So H-S, et al. β-Lapachone suppresses the lung metastasis of melanoma via the MAPK signaling pathway. PLOS ONE2017, 12(5): e0176937.
  2. Sang M., Zhang J., Zhuge Q. Selective cytotoxicity of the antibacterial peptide ABP--Cecropin A and its analog towards leukemia cells, European Journal of Pharmacology2017, 803, 138-147.
  3.  Khedgikar V, Abbruzzese G, Mathavan K, Szydlo H, Cousin H, Alfandari D. Dual control of pcdh8l/PCNS expression and function in Xenopus laevis neural crest cells by adam13/33 via the transcription factors tfap2α and arid3a. eLife, 2017; 6: e26898.
  4. Xu, J., Sun, Y., Li, Y., Ruthel, G., Weiss, S.R., Raj, A., Beiting, D. and Lopez, C. Replication Defective Viral Genomes Exploit A Cellular Pro-Survival Mechanism To Establish Viral Persistence. bioRxiv, 2017, 120766.
  5. Sun, Y. and López, C. B. Preparation of respiratory syncytial virus with high or low content of defective viral particles and their purification from viral stocks. Bio-Protocol 2016, 6(10): e1820.
  6. Xue J, Chambers BS, Hensley SE, López CB. Propagation and Characterization of Influenza Virus Stocks That Lack High Levels of Defective Viral Genomes and Hemagglutinin Mutations. Frontiers in Microbiology2016, 7, 326.
  7. Sun, Y., Jain, D., Koziol-White, C. J., Genoyer, E., Gilbert, M., Tapia, K., Panettieri, R. A., Hodinka, R. L. and Lopez, C. B. Immunostimulatory Defective Viral Genomes from Respiratory Syncytial Virus Promote a Strong Innate Antiviral Response during Infection in Mice and Humans. PLOS Pathogens2015, 11(9), e1005122.
  8. Sun G-G, Wang Y-D, Cui D-W, Cheng Y-J, Hu W-N. Epithelial membrane protein 1 negatively regulates cell growth and metastasis in colorectal carcinoma. World Journal of Gastroenterology2014, 20, 4001-4010.
  9. Pincus S., Moran E., Maresh G., Jennings H. J., Pritchard, D. J., Egan M. L, Blixt O., Fine specificity and cross-reactions of monoclonal antibodies to group B streptococcal capsular polysaccharide type III, Vaccine2012, 30, 4849-4858.
  10. Uphoff C., Drexler H., Elimination of mycoplasmas from infected cell lines using antibiotics. Methods in Molecular Biology2011, 731: 105-114.
  11. Bürdek, M., Spranger, S., Wilde, S., Frankenberger, B., Schendel, D. J., Geiger, C. Three-day dendritic cells for vaccine development: Antigen uptake, processing and presentation. Journal of Translational Medicine2010, 8, 90.
  12. Conroy A, Serghides L, Finney C, Owino SO, Kumar S, Gowda C, Liles C, Moore J, Kain K. C5a enhances dysregulated inflammatory and angiogenic responses to malaria in vitro: potential implications for placental malaria. PLOS ONE 2009, 4: e4953.
  13. W. Mnif, M.J. Duchesne, P. Balaguer, J.C. Nicolas, V. Cavaillès, A. Bartegi, E. Badia, Efficient treatment of Mycoplasma hyorhinis contamination in MCF-7 breast cancer cells with doxycycline. Immuno-Analyse & Biologie Spécialisée, 2007, 22, 107-110.
  14. Uphoff C, Drexler H. Eradication of mycoplasma contaminations., Methods Molecular Biology 2005, 290, 25-34.
  15. McKimm-Breschkin J, A simplified plaque assay for respiratory syncytial virus--direct visualization of plaques without immunostaining., Journal of Virological Methods2004, 120, 113-117.
  16. Uphoff C, Drexler H. Comparative antibiotic eradication of mycoplasma infections from continuous cell lines. In Vitro Cellular & Development Biology-Animal2002, 38, 86-89.
  17. Rowea J, Scraggb I, Kwiatkowski D, Ferguson D, Carucci D, Newbold C, Implications of mycoplasma contamination in cultures and methods for its detection and eradication. Molecular and Biochemical Parasitology, 1998, 92, 177-180.
  18. Macfarlane, D., Dahle C. Isolating RNA from whole blood—the dawn of RNA-based diagnosis? Nature1993, 362, 186-188.

 

Products included in this offer:

SKU Product Name Size Promo List Price Sale Price Availability Quantity
093050044 MYCOPLASMA REMOVAL AGENT 5 ml - $280.90 - In Stock
093030000 MYCOPLASMA STAIN KIT 1 kit - $503.60 - In Stock