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Penetratin™: your vector for non-traumatic internalization of hydrophilic compounds into Eukaryotic cells
Penetratin™ is a patented 16-amino acid peptide corresponding to the third helix homeodomain of Drosophila Antennapedia (pAntp) protein. This peptide is able to translocate across biological membranes by an energy-independent mechanism.
With the use of Penetratin™ peptide, covalently attached peptides, Oligonucleotides and siRNA are internalized and conveyed to the cytoplasm and nucleus in a wide variety of cell lines.
The activated Penetratin™ Peptide features a pyridyl disulfide function at its N-terminal end. The molecule to be coupled must have a free thiol group (-SH) available (i.e. a cysteine in the case of peptides).
E18 primary rat hippocampal neurons were treated with siRNA targeting the X-linked inhibitor of apoptosis protein (XIAP) alone (left) or complexed to Penetratin™ 1 (right). 24 hours later RNAi was detected by staining for XIAP (green). Cells were stained for cytochrome c as a control (red).
Note:1 Courtesy of Dr. C.M. Troy, Columbia University Medical Center, New York. The Journal of Neuroscience, November 10, 2004 • 24(45):10040 - 10046
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