Tetsuya Harada, Takao Kawai, Michio Jinnai, Takahiro Ohnishi, Yoshiko Sugita-Konishi and Yuko Kumeda.
Division of Bacteriology, Osaka Prefectural Institute of Public Health, Osaka, Japan,and Division of Microbiology,
National Institute of Health Sciences, Tokyo, Japan.

Overview

  • Key words: Tissue biopsy, medical microbiology, bacterial identification
  • Aim of the study: Isolation of microorganisms from infected biopsy specimens
  • Application: Bacterial culture
  • Sample name: Tissue biopsy
  • Material: FastPrep-24™ instrument, TeenPrep sample holder, 8-10 ceramic beads (Lysing Matrix M, ΒΌ ")
  • Buffer: glucose broth

Protocols & Parameters

Tissue biopsies are transferred with tweezers from original glass and into 15 ml sterile centrifuge tube (use 2 ml tube if very small biopsy).
  1. Add between 1 g and 2 g biopsy sample to a 15 ml Lysing Matrix tube containing 8-10 large 1/4 inch ceramic beads.
  2. Add 5ml glucose broth.
  3. Homogenize samples in the FastPrep-24™ instrument for 40 seconds at a speed setting of 6.5 m/s.
  4. After homogenizing, take out the tube(s) from the FastPrep-24™ instrument and remove the beads.
  5. Inoculate media and transfer the homogenized biopsy to a sterile glass for storage.

Conclusion

  • The FastPrep® System (FastPrep-24™ instrument, TeenPrep sample holder, Lysing Matrix M) provides effective homogenization of tissue biopsies and did not result in cross-contamination of the samples.
  • The culture results were proven better than the manual method.