Sample Prep Solutions
Learn more about the complete FastPrep® sample prep solution.
Lysing Matrix Tubes
Many Optimized FastPrep Matrices to Lyse Even your Toughest Samples.See All Matrix Options
Ergonomic design ensures easy loading and secure homogenization.
DNA & RNA Extraction Kits
Delivering superior quality DNA, RNA and Proteins for any downstream application.
Sample Prep Solutions for Wastewater
Quickly Extract the Cleanest DNA and RNA from Wastewater
Isolating DNA or RNA from wastewater and sludge is difficult because of the high concentrations of PCR inhibiting compounds such as humic and fulvic acids, and polyphenols. The FastDNA Spin Kit for Soil and FastRNA Pro Soil Kits will help you overcome any difficulties with complete lysis of water-borne environmental organisms including historically difficult sources such as eubacterial spores and endospores, gram positive bacteria, yeast, algae, fungi, resulting in the isolation of pure DNA and RNA.
Solutions for Isolation of Nucleic Acids from Soil
FastPrep Instruments and Soil Kits are designed for the simple and rapid isolation of humic-free, PCR-quality nucleic acids from microbes water or sludge. They can be used to isolate DNA from tough-to-lyse bacteria, fungi, protozoa, algae and other organisms that inhabit a variety of samples including still and running water, wastewater, sludge, and marine sediments.
First, water-borne organisms are rapidly and efficiently lysed by bead beating using specialized lysing matrix particles and the FastPrep instruments. Then GeneClean Binding Matrix, and spin column plate technology is used to isolate the nucleic acids, which is subsequently filtered to remove humic acids, polyphenols and polysaccharides that can inhibit downstream PCR applications. This procedure can be performed in minutes, and there is no need for organic denaturants or proteinases.
Superior DNA Yields with MP Bio's FastDNA Spin Kit for Soil
The FastDNA Spin Kit for Soil give consistently higher yields of genomic DNA from sludge, water, and other environmental samples. The Lysing Matrix E Tubes supplied in the kit quickly lyse any bacteria, protists, fungi, yeast, plant or animal material found up to a 5.0 mL water sample. The large binding capacity of GenClean GlassMilk matrix allows for extremely high yields, sometimes up to 40 mg of genomic DNA. The image to the right shows a comparison of the FastDNA Spin Kit for Soil vs a top competitor.
Typical Wastewater Sample Recommendations
Publications and References
Alexandrio M, Grohmann E, Szewzyk U. Optimization of PCR-based methods for rapid detection of Campylobacter jejuni, Campylobacter coli and Yersinia enterocolitica serovar 0:3 in wastewater samples. Water Research. 2004; 38:1340-1346.
Caccio SM, De Giacomo M, Aulicino FA, Pozio E. Giardia cysts in wastewater treatment plants in Italy. Appl Environ Microbiol. 2003; 69(6) 3393-3398.
Webster, G. et al.; Asessment of Bacterial Community structure in the deep sub-seafloor biosphere by 16S rDNA-based techniques: a cautionary tale. Journ. of Microbio. Meth. 2003; 55: 155-65