Sample Prep Solutions
Learn more about the complete FastPrep® sample prep solution.
DNA & RNA Extraction Kits
Delivering superior quality DNA, RNA and Proteins for any downstream application.
Lysing Matrix Tubes
Many Optimized FastPrep Matrices to Lyse Even your Toughest Samples.See All Matrix Options
The Most Advanced Sample Preparation Systems Available!
Ergonomic design ensures easy loading and secure homogenization.
Sample Prep Solutions for Tissue
DNA and RNA from Some of Nature's Toughest Samples
Some animal and human tissues are the toughest samples to isolate high-quality DNA or RNA. Hard Samples like bone and tumors, and more elastic samples like skin, can be difficult to lyse with other homogenization systems, but the FastDNA Spin Kit, FastDNA Spin Kit for Plant and Animal Tissue, and the FastRNA Pro Green Kit used in combination with the FastPrep instrument will quickly and efficiently extract PCR-ready DNA and RNA.
FastPrep-24 and Lysing Matrix for use for metabolomic studies
Dr. Jackob M. Haus, M.D. from Cleveland Clinic
Solutions for Isolation of Nucleic Acids from Tissue
The FastDNA Spin Kit for Animal Tissue quickly and efficiently isolates genomic DNA from a wide variety of animal sources. Designed for use with the FastPrep® Instruments from MP Biomedicals, animal tissues, are easily lysed within 40 seconds. These benchtop devices use a unique, optimized motion to homogenize samples by multidirectional, simultaneous impaction with lysing matrix particles.
FastPrep® Instruments provide an extremely quick, efficient and highly reproducible homogenization that surpasses traditional extraction methods. Samples are placed into 2.0 ml tubes containing the proprietary Lysing Matrix D, which are 1.4mm ceramic spheres. While almost all samples are easily processed with Lysing Matrix D, additional lysing matrix options are available for more delicate samples or for tough, hardened samples.
Homogenization of human and animal tissues in the FastPrep® Instrument with Lysing Matrix D takes place in the presence of sample-specific Cell Lysis Solution, CLS-TC. For maximum flexibility, all buffers are provided in the kit. Following lysis, samples are centrifuged to pellet debris and lysing matrix. DNA is purified from the supernatant with a silica-based GENECLEAN® procedure using SPIN filters. Eluted DNA is ready for digestion, electrophoresis, PCR and any other desired application.
RNA 2100 Bioanalyzer analysis of FastPrep sample. The RNA was run on an Agilent 2100 Bioanalyzer (Agilent, Palo Alto, Calif.) using the RNA 6000 Pico LabChip kit to determine the quality of the samples. The 28S and 18S ribosomal bands show a greater than 2:1 ratio, and the calculated RNA ribosomal integrity numbers of the samples ranged from 8.4 to 8.9, verifying high-quality RNA. Shown above are the gel images for 11 RNA samples. Below are two representative electrophoretic graphs showing the RNA peaks.Case Study, Dual Extraction of RNA and Proteins from Skin
Current translational human studies are moving in the direction of concurrent genomic and proteomic analysis using small clinical samples. Two mechanical disruption methods were tested for their efficiency in disrupting biopsy skin samples: the FastPrep System and a Polytron® 1300D homogenizer. The quantity and quality of RNA from both extraction techniques were calculated using a NanoDrop® spectrophotometer. The average yield of 1.4 µg RNA with the FastPrep System was 57% higher than with the Polytron. To verify the high-quality nature of the RNA extracted with the FastPrep system, processed samples were analyzed with the Agilent 2100 Bioanalyzer. All the samples processed with the FastPrep System had ribosomal integrity numbers of between 8.4 and 8.9, which is consistent with high-quality RNA. Protein quantitation was determined using a Coomassie-based assay using a standard containing bovine serum albumin in 8 M urea and 10 mM dithiothreitol. The average yield using the FastPrep System was 170 µg of protein, a 53% increase over the Polytron technique. The quality of extracted proteins was assessed by two dimensional gel and Western blot analysis.
Typical Tissue Sample Recommendations
FastPrep Kits & Instruments
Publications and References
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