Sample Prep Solutions
Learn more about the complete FastPrep® sample prep solution.
DNA & RNA Extraction Kits
Delivering superior quality DNA, RNA and Proteins for any downstream application.
Lysing Matrix Tubes
Many Optimized FastPrep Matrices to Lyse Even your Toughest Samples.See All Matrix Options
The Most Advanced Sample Preparation Systems Available!
Ergonomic design ensures easy loading and secure homogenization.
Sample Prep Solutions for Soil
Quickly and Effectively Turn the Dirtiest into the Cleanest
The challenge with extractions from soil is isolating DNA or RNA without contamination by humic acids or other PCR inhibitors. The FastDNA Spin Kit for Soil and FastRNA Pro Soil Kits will help you overcome any difficulties with complete lysis of all soil organisms including historically difficult sources such as eubacterial spores and endospores, gram positive bacteria, yeast, algae, nematodes and fungi, and isolation of pure DNA and RNA.
Solutions for Isolation of Nucleic Acids from Soil
FastPrep Instruments and Soil Kits are designed for the simple and rapid isolation of humic-free, PCR-quality nucleic acids from microbes in soil. They can be used to isolate DNA from tough-to-lyse bacteria, fungi, protozoa, algae and other organisms that inhabit a variety of samples including clay, sandy, silty, peaty, chalky, and loamy soils. First, soil organisms are rapidly and efficiently lysed by bead beating using specialized lysing matrix particles and the FastPrep instruments. Then GeneClean Binding Matrix, and spin column plate technology is used to isolate the nucleic acids, which is subsequently filtered to remove humic acids, polyphenols and polysaccharides that can inhibit downstream PCR applications. This procedure can be performed in minutes, and there is no need for organic denaturants or proteinases.
FastPrep-24 for metegenomics DNA extraction from soil
Mr. Eric Dunford, graduate student from University of Waterloo is using FastPrep-24 system and kits for DNA extraction and cDNA library construction from hard to lyse soil organisms, from thousands of different soil samples.
Wells from the right: 1) Empty well 2) Ladder 1 3) Ladder 2 4) DNA Sample A using FastDNA soil kit 5) DNA Sample B using FastDNA soil kit 6) DNA sample A using a kit from a top competitor 7) DNA sample B using a kit from a top competitor
Superior DNA Yields with MP Bio's FastDNA Spin Kit for Soil
The FastDNA Spin Kit for Soil give consistently higher yields of genomic DNA from soil, sludge, water, feces and other environmental samples. The Lysing Matrix E Tubes supplied in the kit quickly lyse any bacteria, protists, fungi, yeast, plant or animal material found up to a 500 mg soil sample. The large binding capacity of GenClean GlassMilk matrix allows for extremely high yields, sometimes up to 40 mg of genomic DNA. The image to the right shows a comparison of the FastDNA Spin Kit for Soil vs a top competitor.
Typical Soil Sample Recommendations
FastPrep Kits & Instruments
Publications and References
Dilly, O., et al., (2004) Bacterial Diversity in Agricultural Soils during Litter Decomposition. Applied and Environmental Microbiology 70, 468-74.
Dionisi, H., et al., (2004) Abundance of Dioxygenase Genes Similar to Ralstonia sp. Strain U2 nagAc Is Correlated with Napthalene Concentrations in Coal Tar-Contaminated Freshwater Sediments. Applied and Environmental Microbiology 70, 3988-3995.
Crawford, R., et al., (2002) Measurement of microbial activity in soil by colorimetric observation of in situ dye reduction: an approach to detention of extraterrestrial life. BMC Microbiol. 2.
Fredslund, L., et al., (2001) Development and Application of a Most-Probable-Number-PCR Assay To Quantify Flagellate Populations in Soil Samples. Applied and Environmental Microbiology 67, 1613-1618.
Borneman, J., et al., (2000) PCR Primers that Amplify Fungal rRNA Genes from Environmental Samples. Applied and Environmental Microbiology 6, 4356-4360.
Joulian, C., et al., (2001) Congruent Phylogenies of Most Common Small- Subunit rRNA and Dissimilatory Sulfite Reductase Gene Sequences Retrieved from Estuarine Sediments. Applied and Environmental Microbiology 67, 3314-3318.
Lipthay, J., et al., (2003) In Situ Exposure to Low Herbicide Concentrations Affects Microbial Population Composition and Catabolic Gene Frequency in an Aerobic Shallow Aquifer. Applied and Environmental Microbiology 69, 461-67.
Lueders, T., et al., (2002) Friedrich. Effects of Amendment with Ferrihydrite and Gypsum on the Structure and Activity of Methanogenic Populations in Rice Field Soil. Applied and Environmental Microbiology 68, 2484-2494.
Myers, M., et al., (2003) PCR Detection of a Newly Emerged Pandemic Vibrio parahaemolyticus O3:K6 Pathogen in Pure Cultures and Seeded Waters from the Gulf of Mexico. Applied and Environmental Microbiology 69, 2194-2200.
Newberry, C., et al., (2004) Diversity of prokaryotes and methanogenesis in deep subsurface sediments from the Nankai Trough, Ocean Drilling Program Leg 190. Applied and Environmental Microbiology 6, 274-87.
Feng, Y., et al., (2009) Free-air CO2 enrichment (FACE) enhances the biodiversity of purple phototrophic bacteria in flooded paddy soil. Plant Soil 324, 317-328.
Fyfe, JAM., et al., (2007) Development and Application of Two Multiplex Real-Time PCR Assays for the Detection of Mycobacterium ulcerans in Clinical and Environmental Samples. Applied and Environmental Microbiology 73, 4733-40.
Ibekwe A.M., et al., (2001) Impact of Fumigants on Soil Microbial Communities. Applied and Environmental Microbiology 67, 3245?3257.
Pan, Y., et al., (2010) Impacts of Inter- and Intralaboratory Variations on the Reproducibility of Microbial Community Analyses. Applied and Environmental Microbiology 76, 7451-58.
Hultman, J., et al. (2008) Universal ligation-detection-reaction microarray applied for compost microbes. BMC Microbiology 8.
Dedysh, S.N., et al. (2006) Phylogenetic Analysis and In Situ identification of Bacteria Community Composition in an Acidic Sphagnum Peat Bog. Applied and Environmental Microbiology 72, 2110-2117.
Weinert, N., et al. (2009) Rhizosphere Communities of Genetically Modified Zexanthin-Accumulating Potato Plants and Their Parent Cultivar Differ Less than Those of Different Potato Cultivars. Applied and Environmental microbiology 75, 3859-3865.
Mirete, S., et al. (2007) Novel Nickel Resistance Genes from the Rhizosphere Metagenome of Plants Adapted to Acid Mine Drainage. Applied and Environmental microbiology 73, 6001-6011.
Castaldini, M., et al. (2005) Impact of Bt Corn on Rhizospheric and Soil Eubacterial Communities and on Beneficial Mycorrhizal Symbiosis in Experimental Microcosms. Applied and Environmental microbiology 71, 6719-6729.