Sample Prep Solutions
Learn more about the complete FastPrep® sample prep solution.
DNA & RNA Extraction Kits
Delivering superior quality DNA, RNA and Proteins for any downstream application.
Lysing Matrix Tubes
Many Optimized FastPrep Matrices to Lyse Even your Toughest Samples.See All Matrix Options
The Most Advanced Sample Preparation Systems Available!
Ergonomic design ensures easy loading and secure homogenization.
Sample Prep Solutions for Plants
Pretty to Look at, Tough to Lyse
Because plant samples can be very fibrous and contain high levels of polyphenolic compounds, polysaccharides, and RNases, it can be extremely difficult to extract enough usable DNA or RNA for PCR analysis and other downstream applications. The FastDNA Kit, FastDNA Spin Kit, FastRNA Pro Green Kit and FastDNA-96 Plant and Seed DNA Kit coupled with the FastPrep® Instruments make it fast and simple!
Sample Preparation Challenge-Lysis of Plant Tissue
The FastPrep®System, with optional large volume adapters and lysing matrix tubes, is the perfect sample preparation system for the extraction and purification of nucelic acids and proteins from plant tissue.
Plant tissue poses many challenges during sample preparation procedures, due to fibrous tissue in leaves, roots and stems, tough seedcoats of grains, and an abundance of PCR inhibitors. All of these samples are quickly and efficiently lysed with FastPrep®.
The FastDNA Spin Kit for Plant and Animal Tissue effectively removes PCR inhibitors such as tannins, humic substances, polyphenols, and polysaccarhides and results in pure DNA that is ready for a host of downstream operations with no further purification necessary. The FastPrep Systems can also be used to isolate nucleic acids and proteins from plant pathogens such as bacteria, yeast, fungi, and invasive cysts and spores.
High-Throughput Solutions for Difficult-to-Lyse Plant Samples
The FastDNA-96 Plant & Seed DNA Kit quickly and efficiently isolates inhibitor-free, PCR-ready genomic DNA from up to 192 plant samples simultaneously in approximately 50 minutes. Tough-to-lyse plant samples including stems, roots, leaves, buds, flowers, fruits and seeds are efficiently lysed in approximately 60 seconds with the FastPrep-96 Instrument and the kit includes all buffers necessary complete DNA purification. The MP-96 Inhibitor Removal Plate included effectively eliminates PCR inhibitors, polyphenols/tannins, and polysaccharides from up to 80 mg samples, resulting in highly purified DNA (A260/A280 ratios = 1.8) that is ready for all downstream application.
Typical Plant Sample Recommendations
FastPrep Kits & Instruments
Publications and References
Brown GR, Kadel III EE, Bassoni DL, Temesgen B, Van Buijtenen JP, Sewell MM, Marshall KA, Neale DB. Anchored reference loci in loblolly pine (Pinus taeda L.) for integrating pine genomics. Genetics 2001; 159:799-809.
Calderon C, Ward E, Freeman J, Foster SJ, McCartney HA. Detection of airbone inoculum of Leptosphaeria maculans and Pyrenopeziza brassicae in oilseed rape crops by polymerase chain reaction (PCR) assays. Plant Pathol. 2002; 51:303-10.
Cano J, Guarro J, Gene J. Molecular and Morphological Identification of Colletotrichum Species of Clinical Interest. Journ. Clin. Microbiol. 2004; 42(6):2450-2454.
Erkkilä MJ, Leah R, Ahokas H, Cameron-Mills V. Allele-dependent Barley Grain beta amylase activity. Plant Physiol. 1998; 117:679-85.
Eisenbarth D, Weig, A. Dynamics of aquaporins and water relations during hypocotyl elongation in Ricinus during hypocotyl elongation in Ricinus. Journ. Exp. Bot. 2005; 56(417):1831-1842.
Franco-Zorrilla J, Cubas P, Jarillo J, Fernandez-Calvon, B, Salinas J, Martinez-Zapater J. AtREM1, a Member of a New Family of B3 Domain-Containing Genes, Is Preferentially Expressed in Reproductive Meristems. Plant Physiol. 2002; 128:481-427.
Frewen B, Chen T, Howe G, et al. Quantitative Trait Loci and Candidate Gene Mapping of Bud Set and Bud Flush in Populus. Genetics 2000; 154:837-45.
Jensen C, Salchert K, Nielsen K. A TERMINAL FLOWER1-Like Gene from Perennial Ryegrass Involved in Floral Transition and Axillary Meristem Identity. Plant Physiol. 2001; 125:1517-1528.
Long J, Zhao W, Rashotte A, Muday G, Huber. Gravity-Stimulated Changes in Auxin and Invertase Gene Expression in Maize Pulvinal Cells. Plant Physiol. 2002; 128:591-602.
Bao JR, Lazarovits G. Evaluation of three procedures for recovery of GUS enzyme and colony forming units of a nonpathogenic strain of Fusarium oxysporum, 70T01, from inoculated tomato roots. Can. J. Plant Pathol. 2002; 24:340?348
Eisenbarth, DA, Weig AR. Dynamics of aquaporins and water relations during hypocotyl elongation in Ricinus communis L. seedlings. Journ. of Exp. Botany 2005; 56(417):1831?1842
Haymes KM. et al., Rapid Isolation of DNA from Chocolate and Date Palm Tree Crops. J. Agric. Food Chem. 2004; 52:5456-5462
Jensen CS. et al., A TERMINAL FLOWER1-Like Gene from Perennial Ryegrass Involved in Floral Transition and Axillary Meristem Identity. Plant Physiology 2001; 125:1517?1528
Yang CH. et al., Microbial phyllosphere populations are more complex than previously realized. PNAS 2001; 98(7):3889?3894