Sample Prep Solutions

Learn more about the complete FastPrep® sample prep solution.

Lysing Matrix Tubes

Many Optimized FastPrep Matrices to Lyse Even your Toughest Samples.

See All Matrix Options
FastPrep Insturments

The Most Advanced Sample Preparation Systems Available!

FastPrep Adapters

Ergonomic design ensures easy loading and secure homogenization.

Lyse any microbial cells with ease for the best extractions
One of the primary challenges with lysis of yeasts is that it can be very tough, especially in the form of spores. Same challenge applies to most of bacterial samples that can be very tough, especially if you are working with gram positive or gram negative bacteria or spores. With the FastPrep® Instruments, optimized lysing matrix kits, and our optimized FastDNA™ Spin Kit, and the FastRNA™ Spin Kits, isolating DNA and RNA from yeasts and microbes is fast and simple.
FastPrep Systems Application in Astrobiology

Dr. Moogega Cooper. Ph.D., a CalTech Postdoctotal Scholar at the NASA Jet Propulsion Labotatory.

Lysis of microbial cells in FastPrep-24
Lyse any microbial cells with ease for the best extractions

FastPrep-24™ and its larger cousin, FastPrep-96™ are the most optimal solutions for reproducible and safe sample preparation of microbiological samples.

Using optimized Lysing Matrix B for bacteria, Lysing Matrix C for yeast and Lysing Matrix E for fecal and environmental sampleyou can expect consistent quality and quantity of your lysate each experiment-every time! All of your microbial samples can be processed, using optional adapters in variety of sample sizes from 2ml up to 50ml tubes in FastPrep-24™ and from 96 well plates up to 250 ml tubes in FastPrep-96™

Hundreds of articles describe the use of FastPrep systems and associated FastDNA and FastRNA family of kits, for the extraction of DNA and RNA from even the most difficult microorganisms such as mycobacteriums and anthrax spores or from most difficult and dirty matrixed samples, such as a mouse turd, deep marine sediments, archeological and paleontological samples etc..

In the case of soil or fecal microbial DNA extraction you can expect to have entire metagenomics, i.e. quantitative extraction of genomic materials from entire microbial populations. For the RNA extractions and for extractions of the enzyme and metabolites you can significiantly increase the yield by using some of our CoolPrep™ cryogenic adapters to lower lysis temperature. FastPrep systems and FastProtein™ Blue and Red kits are ideal for producing solution for an entire proteomics lysate with minimal degradation of higher structures even for the unstable enzymes and proteins.

Aqarose gel electrophoresis with ethidium bromide staining FastPrep

Lanes 1 to 8 correspond to the samples processed in the FastPrep: 1 to 4 are at 0.4 mg/ml and 5 to 8 at 0.1 mg/ml. 1 and 5 at time 0, 2 and 6 at 40 s, 3 and 7 at 2 x 40s, 4 and 8 are 3 x 40 s. Lanes 9, 10 and 11 correspond to sonication samples (S) taken at 4 x 40 s, 7 x 40 s and 9 x 40 s, respectively. Lane 12: ? DNA cut by purified Bam HI (C).

Case Study- Protein Extraction from Gram-positive Bacteria

The experimental data presented above clearly show that the FastPrep instrument using FastProtein Blue matrix can be used to successfully extract unstable enzymes from gram-positive bacteria. Even in cases where sonication can release active materials (such as the Bacillus amyloliquefaciens experiments here), the lysing time can be reduced by approximately 60%.

For samples like Staphylococcus aureus 3A that require longer and less efficient methods of lysis (such as French Press), the FastPrep method offers clear advantages for extraction of active proteins.

Lyse any microbial cells with ease for the best extractions
Sample Name Sample Type Quantity Lysing Matrix FastPrep Speed FastPrep Time
Escherichia coli Cells (Bacteria) 108 Cells B 6.0 40 sec
Lactococcus lactis Cells (Bacteria) 108 Cells B 6.0 40 sec
Listeria monocytogenes Cells (Bacteria) 108 Cells B 6.0 2 x 40 sec
Mycobacterium tuberculosis Cells (Bacteria) 108 Cells B 6.0 40 sec
Streptococcus mutans Cells (Bacteria) 108 Cells B 6.0 40 sec
Streptococcus pyogenes Cells (Bacteria) 108 Cells B 6.0 40 sec
Photorhabdus luminescens Cells (Bacteria) 108 Cells B 6.0 40 sec
Aspergillus fumingatus Cells (Fungi) 108 Cells C 6.0 2 x 30 sec
Acandida albicans Cells (Fungi) 108 Cells C 6.0 2 x 30 sec
Cryptococcus neoformans Cells (Fungi) 108 Cells C 6.0 4 x 30 sec
Fusarium solani Cells (Fungi) 108 Cells C 6.0 2 x 30 sec
Saccharomyces cerevisiae Cells (Fungi) 2 x 108 Cells C 6.0 40 sec
Schizosaccharomyces pombe Cells (Fungi) 108 Cells C 6.0 4 x 15 sec
Schizosaccharomyces pombe Cells (Fungi) 108 Cells B 6.0 40 sec
FastPrep Kits & Instruments
Purification Kits SKU Free Sample (Availability)
FastDNA™ SPIN KIT 116540000
FastRNA™ SPIN KIT 116025050 N/A
Isolating DNA and RNA from yeasts 116540000
116030050 N/A
FastProtein™ Blue and Red kits 116550400 N/A
116550600 N/A
Bacteria Publications and References
Abeyta M, Hardy GG, Yother J. Genetic alteration of capsule type but not PspA type affects accessibility of surface-bound complement and surface antigens of Streptococcus pneumoniae. Infect Immun. 2003; 71(1):218-25.
Bekri MA, Desair J, Keijers V, et al. Azospirillum irakense produces a novel type of pectate lyase. J Bacteriol. 1999; 181(8):2440-7.
Belley A, Alexander D, Di Pietrantonio T, et al. Impact of methoxymycolic acid production by Mycobacterium bovis BCG vaccines. Infect Immun. 2004;72(5):2803-9.
Borneman J, Skroch PW, O'Sullivan KM, et al. Molecular microbial diversity of an agricultural soil in Wisconsin. Appl Environ Microbiol. 1996; 62(6):1935-43.
Filipe SR, Pinho MG, Tomasz A. Characterization of the murMN operon involved in the synthesis of branched peptidoglycan peptides in Streptococcus pneumoniae. J Biol Chem. 2000; 275(36):27768-74.
Fontaine MC, Lee JJ, Kehoe MA. Combined contributions of streptolysin O and streptolysin S to virulence of serotype M5 Streptococcus pyogenes strain Manfredo. Infect Immun. 2003; 71(7):3857-65.
Gieseke A, Purkhold U, Wagner M, Amann R, Schramm A. Community structure and activity dynamics of nitrifying bacteria in a phosphate-removing biofilm. Appl Environ Microbiol. 2001; 67(3):1351-62.
Gravesen A, Ramnath M, Rechinger KB, et al. High-level resistance to class IIa bacteriocins is associated with one general mechanism in Listeria monocytogenes. Microbiology. 2002; 148(Pt 8):2361-9.
Handke LD, Conlon KM, Slater SR, et al. Genetic and phenotypic analysis of biofilm phenotypic variation in multiple Staphylococcus epidermidis isolates. J Med Microbiol. 2004; 53(Pt 5):367-74.
Kocianova S, Vuong C, Yao Y, et al. Key role of poly-gamma-DL-glutamic acid in immune evasion and virulence of Staphylococcus epidermidis. J Clin Invest. 2005; 115(3):688-94.
Yeast Publications and References
Albers E, Laize V, Blomberg A, Hohmann S, Gustafsson L. J Biol Chem. 2003; 278(2): 10264-10272.
Borneman J, Hartin RJ. PCR Primers That Amplify Fungal rRNA Genes from Environmental Samples. Appl Environ Microbiol. 2000; 66(10): 4356-4360.
Bro C, Regenberg B, Laginel G, Labarre J, Montero-Lomeli M, Nielsen J. J Biol Chem. 2003; 278(34): 32141-32149.
Capasso H, Palermo C, Wan S, Rao H, John UP, O'Connell MJ, Walworth NC. J Cell Sci. 2002; 115: 4555-4564.
Gojkovic Z, Sandrini M, Pis'kur J. Eukaryotic -Alanine Synthases Are Functionally Related but Have a High Degree of Structural Diversity. Genetics. 2001; 158:999-1011.
Goovis G, Yarden O. Environmental Suppression of Neurospora crassa cot-1 Hyperbranching: a Link between COT1 Kinase and Stress Sensing. Eukary. Cell. 2003; 2(4):699-707.
Hohl M, Christensen O, Kunz C, Naegeli H, Fleck. Binding and Repair of Mismatched DNA Mediated by Rhp14, the Fission Yeast Homologue of Human XPA. J Biol Chem. 2001; 276(33):30766-30772.
Tsai H, Bard M, Izumikawa K, Krol A, Sturm A, Culbertson N, Pierson C, Bennett J. Candida glabrata erg1 Mutant with Increased Sensitivity to Azoles and to Low Oxygen Tension. Antimicrob Agents Chemother. 2004; 48(7):2483-2489.
Lu J, Pollard T. Profilin Binding to Poly-L-Proline and Actin Monomers along with Ability to Catalyze Actin Nucleotide Exchange Is Required for Viability of Fission Yeast. Molec Biol Cell. 2001; 12:1161-1175.
Hendrickson E, Payne J, Young R, et al. Molecular Analysis of Dehalococcoides 16S Ribosomal DNA from Chloroethene-Contaminated Sites throughout North America and Europe. Appl Environ Microbiol. 2002; 68(2):485-495.