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Ham's F10 (10X Solution) without L-Glutamine, without Sodium Bicarbonate, with 12.4 mg/L Phenol Red

Catalog Number: 1440049, 1440054
Ham's F10 (10X Solution) without L-Glutamine, without Sodium Bicarbonate, with 12.4 mg/L Phenol Red


Description: Originally, Ham's Nutrient Mixtures were developed for the clonal growth of several Chinese hamster ovary (CHO) clone cells, Hela cells and mouse L-cells. Both F-10 and F-12 are formulated for use with or without serum, depending on the type of cells being cultured. F-10 has demonstrated satisfactory growth of human diploid cells, white blood cells for chromosomal analysis and primary rat, rabbit and chicken tissue explants. Ham's F-12 is well suited for the growth of primary rat hepatocytes and prostate epithelial cells. It is also the medium of choice for toxicity assays using CHO cells. MP also offers Ham's F-12 supplemented with 25 mM HEPES for improved buffering in the optimum pH range of 7.2-7.4.

F12K is a modification of Ham's F-12 and Coon's F-12 with increased amino acid and pyruvate concentrations. Additionally, the salts have been modifed (Konigsbergs). F12K has been developed for culturing differentiated rat and chicken cells, as well as, primary human liver cells.

References:

  1. Ham, R.G., Exptl. Cell Res., v. 39, 515 (1963).
  2. Morton, H.J., In Vitro, v. 6, 89 (1970).
  3. Rutaky, L.P. and Pumper, R.W., In Vitro, v. 9, 648 (1974).
  4. Soloman, J.J., TCA manual, v. 1, 7 (1975).

MgCl2 has been substituted by MgSO4 containing an equivalent amount of Mg ion.

Formulation (as 10X):
Component
mg/lt
Mol. Wt.
Mol. (mM)
Amino Acids
L-Alanine
89.10000
89.1
1.00
L-Arginine HCl
2107.00000
174.2
12.10
L-Asparagine H2O
150.10000
150.1
1.00
L-Aspartic Acid
133.10000
133.1
1.00
L-Cysteine HCl H2O
351.30000
175.6
2.00
L-Glutamic Acid
147.10000
147.1
1.00
Glycine
75.10000
75.07
1.00
L-Histidine HCl H2O
209.60000
209.6
1.00
L-Isoleucine
26.20000
131.2
0.20
L-Leucine
131.20000
131.2
1.00
L-Lysine HCl
293.00000
182.6
1.60
L-Methionine
44.80000
149.2
0.30
L-Phenylalanine
49.60000
165.2
0.30
L-Proline
115.10000
115.1
1.00
L-Serine
105.10000
105.1
1.00
L-Threonine
35.70000
119.1
0.30
L-Tryptophan
6.10000
204.2
0.03
L-Tyrosine
18.10000
181.2
0.10
L-Valine
35.10000
117.1
0.30
Vitamins
Biotin
0.24000
244.3
0.0010
Choline Chloride
6.98000
139.6
0.05
D-Calcium Pantothenate
7.15000
238.3
0.03
Folic Acid
13.20000
441.4
0.03
myo-Inositol
5.41000
180.2
0.03
Nicotinamide
6.11000
122.13
0.05
Pyridoxine HCl
2.06000
205.6
0.01
Riboflavin
3.76000
376.4
0.01
Thiamine HCl
10.12000
337.3
0.03
Vitamin B12
13.60000
1355.4
0.01
Inorganic Salts
Calcium Chloride [CaCl2 2H2O] Dihydrate
441.00000
147
3.00
Cupric Sulfate [CuSO4]
0.01600
159.68
0.0001
Ferrous Sulfate [FeSO4 7H2O] Heptahydrate
8.34
278
0.03
Magnesium Sulfate [MgSO4]
746.00000
120.4
6.20
Potassium Chloride [KCl]
2850.00000
74.55
38.23
Potassium Phosphate Monobasic [KH2PO4]
830.00000
136.09
6.10
Sodium Chloride [NaCl]
74000.00000
58.44
1266.26
Sodium Phosphate Dibasic [Na2HPO4]
1562.00000
141.96
11.00
Zinc Sulfate [ZnSO4 7H2O] Heptahydrate
0.28800
287.5
0.0010
Other
Dextrose
11000.00000
180.2
61.04
Hypoxanthine
40.80000
136.1
0.30
Lipoic Acid
2.06000
206.3
0.01
Phenol Red Sodium Salt
12.40000
376.4
0.03
Sodium Pyruvate
1100.00000
110
10.00
Thymidine
7.27000
242.2
0.03
Add
NaHCO3 Powder (g/L) (for 1X Solution)
1.2000
NaHCO3 7.5% Solution (mL/L) (for 1X Solution)
16.0300
L-glutamine Powder (mg/L) (for 1X Solution)
146.2000
L-glutamine 200 mM Solution (mL/L) (for 1X Solution)
5.0000

Availability:

1X Liquid:

Ham's F-10 Medium:
Catalog NumberDescriptionSize
1240249
1240254
1X Ham's F-10 without L-glutamine100 ml
500 ml
1240349
1240354
1X Ham's F-10 with L-glutamine100 ml
500 ml
1240449
1240454
1X Ham's F-10 with 20 mM HEPES, without L-glutamine, sodium bicarbonate100 ml
500 ml

Ham's F-12 Medium:
Catalog NumberDescriptionSize
12423541X Ham's F-12 with L-glutamine500 ml
12422541X Ham's F-12 without L-glutamine500 ml
12424541X Ham's F-12 Kaighn's Modification500 ml

Dulbecco's Modification of Eagle's Medium/Ham's F-12 Medium:
Catalog NumberDescriptionSize
12467541X DMEM/Ham's F-12 (1:1) without L-glutamine500 ml

10X Liquid:

Ham's F-10 Medium:
Catalog NumberDescriptionSize
144004910X Ham's F-10 without L-glutamine, sodium bicarbonate100 ml

Powders:

Ham's F-10 Medium:
Catalog NumberDescriptionSize
1040120
1040122
1040124
Ham's F-10 with L-glutamine without sodium bicarbonate10 x 1 liter
1 x 10 liter
1 x 50 liter
1040220
1040222
1040222
Ham's F-10 with L-glutamine without sodium bicarbonate, hypoxanthine10 x 1 liter
1 x 10 liter
1 x 50 liter

Ham's F-12 Medium:
Catalog NumberDescriptionSize
1042120
1042122
Ham's F-12 with L-glutamine without sodium bicarbonate10 x 1 liter
1 x 10 liter