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Medium 199 with Earle's Salt (E199), powder, with L-glutamine, without sodium bicarbonate

Catalog Number: 1020120, 1020122
Medium 199 with Earle's Salt (E199), powder, with L-glutamine, without sodium bicarbonate


Description: Medium 199, originally described by Morgan and his colleagues (1950), is a completely defined nutritional source for cell culture. Their investigations demonstrated that cell growth could be measured in this medium. It has broad species applicability including the culturing of non-transformed cell types. It may be used for vaccine production and the in vitro cultivation of rat lens tissues and primary mouse pancreatic epithelial explants. It is available in either Earle's or Hanks' salts.

References:

  1. Morgan, J.F., et. al., Proc. Soc. Exptl. Biol. Med., v. 51, 1 (1950).
  2. Morgan, J.F., et. al., J. Natl. Cancer Inst., v. 16, 557 (1955).
  3. Parker, R.C., In: Methods of Tissue Culture, 3rd Ed., Harper & Row, New York (1961).

Note: The DL-amino acids of the original formulation have been replaced by L-amino acids at half the concentration.

Formulation:
Components
mg/L
Mol. Wt.
Mol. (mM)
Amino Acids
L-Alanine
25.000
89.1
0.28
L-Arginine HCl
70.000
174.2
0.40
L-Aspartic Acid
30.000
133.1
0.23
L-Cysteine HCl H2O
0.110
175.6
0.0006
L-Cystine 2HCl
26.000
313.2
0.08
L-Glutamic Acid
75.000
147.1
0.51
L-Glutamine
100.000
146.1
0.68
Glycine
50.000
75.07
0.67
L-Histidine HCl H2O
21.880
209.6
0.10
L-Hydroxyproline
10.000
131.1
0.08
L-Isoleucine
20.000
131.2
0.15
L-Leucine
60.000
131.2
0.46
L-Lysine HCl
70.000
182.6
0.38
L-Methionine
15.000
149.2
0.10
L-Phenylalanine
25.000
165.2
0.15
L-Proline
40.000
115.1
0.35
L-Serine
25.000
105.1
0.24
L-Threonine
30.000
119.1
0.25
L-Tryptophan
10.000
204.2
0.05
L-Tyrosine 2Na 2H2O
57.870
261.2
0.22
L-Valine
25.000
117.1
0.21
Vitamins
Biotin
0.010
244.3
0.00004
Calciferol
0.100
396.7
0.00025
Choline Chloride
0.500
139.6
0.00358
D-Calcium Pantothenate
0.010
238.3
0.00004
DL-a-Tocopherol PO4 Na2
0.010
554.7
0.00002
Folic Acid
0.010
441.4
0.00002
L-Ascorbic Acid
0.050
176.1
0.00028
Menadione Sodium Bisulfite 3H2O
0.019
330.2
0.00006
myo-Inositol
0.050
180.2
0.00028
Niacin
0.025
123.1
0.00020
Nicotinamide
0.025
122.13
0.00020
para-Aminobenzoic Acid
0.050
137.1
0.00036
Pyridoxine HCl
0.050
205.6
0.00024
Riboflavin
0.010
376.4
0.00003
Thiamine HCl
0.010
337.3
0.00003
Vitamin A Acetate
0.140
328.5
0.00043

Inorganic Salts
Calcium Chloride [CaCl2]
200.000
111
1.80
Ferric Nitrate [Fe(NO3)3 9H2O] Nonahydrate
0.720
404
0.0018
Magnesium Sulfate [MgSO4]
97.700
120.4
0.81
Potassium Chloride [KCl]
400.000
74.55
5.37
Sodium Chloride [NaCl]
6800.000
58.44
116.36
Sodium Phosphate Monobasic [NaH2PO4 H2O] Monohydrate
140.000
159.96
0.88
Other
2-Deoxy-D-Ribose
0.500
134.1
0.0037
5-Adenylic Acid H2O
0.200
347.2
0.0006
Adenine Sulfate
10.000
386.4
0.0259
ATP 2Na 3H2O
1.000
605.2
0.0017
Cholesterol
0.200
386.7
0.0005
Dextrose
1000.000
180.2
5.5494
D-Ribose
0.500
150.1
0.0033
Glutathione (reduced)
0.050
307.33
0.0002
Guanine HCl
0.300
187.55
0.0016
Hypoxanthine Na
0.354
159.08
0.0022
Phenol Red Sodium Salt
10.000
376.4
0.0266
Sodium Acetate
50.000
82.03
0.6095
Thymine
0.300
126.1
0.0024
Tween 80
5.000
n/a
n/a
Uracil
0.300
112.1
0.0027
Xanthine Na
0.340
174.1
0.0020

Add
NaHCO3 Powder (g/L)
2.200
NaHCO3 7.5% Solution (mL/L)
29.400

Availability:

1X Liquid:

Medium 199 with Earle's Salts:
Catalog NumberDescriptionSize
12203541X E199 with L-glutamine500 ml
1220249
1220254
1X E199 without L-glutamine100 ml
500 ml
12204491X E199 with 20 mM HEPES, without sodium bicarbonate, L-glutamine100 ml

Medium 199 with Hanks' Salts:
Catalog NumberDescriptionSize
12233541X H199 with L-glutamine500 ml
1223249
1223254
1X H199 without L-glutamine100 ml
500 ml

10X Liquid:

Medium 199 with Earle's Salts:
Catalog NumberDescriptionSize
1420049
1420054
10X E199 without sodium bicarbonate, L-glutamine100 ml
500 ml

Medium 199 with Hanks' Salts:
Catalog NumberDescriptionSize
142305410X H199 without sodium bicarbonate, L-glutamine500 ml

Powders:

Medium 199 with Earle's Salts:
Catalog NumberDescriptionSize
1020120
1020122
E199 with L-glutamine, without sodium bicarbonate10 x 1 liter
1 x 10 liter

Medium 199 with Hanks' Salts:
Catalog NumberDescriptionSize
1023120
1023122
1023124
H199 with L-glutamine, without sodium bicarbonate10 x 1 liter
1 x 10 liter
1 x 50 liter