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Methionine/Choline Deficient and Control Diets

Catalog Number: 960438, 960439, 960440, 960441
Methionine/Choline Deficient and Control Diets


Description: Nonalcoholic steatohepatitis (NASH) is common liver injury in which the histopathological abnormalities mimic those of alcoholic steatohepatitis. This disorder is most frequently associated with type 2 diabetes, hyperlipidemia, and rapid weight loss.1 Leclercq, et al. published in The Journal of Clinical Investigation, CYP2E1 and CYP4A as microsomal catalysts of lipid peroxides in murine nonalcoholic steatohepatitis, in which they proposed that CYP2E1 could also be a factor in the development of NASH. The mice used in this experiment were fed MP's plain Methionine/Choline and our control diet.

References:

  1. Leclercq, I.A. (Storr Liver Unit, Department of Medicine and Westmead Millennium Institure, University of Sydney at Westmead Hospital, Westmead NSW 2145, Australia), Farrell, G.C. (Storr Liver Unit, Department of Medicine and Westmead Millennium Institure, University of Sydney at Westmead Hospital, Westmead NSW 2145, Australia), Field, J. (Storr Liver Unit, Department of Medicine and Westmead Millennium Institure, University of Sydney at Westmead Hospital, Westmead NSW 2145, Australia), Bell, D.R. (School of Biology, University of Nottingham, University Park, Nottinhgam NG7 2DR, United Kingdom), Gonzalez, F.J. (Laboratory of Molecular Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA) and Robertson, G.R. (Storr Liver Unit, Department of Medicine and Westmead Millennium Institure, University of Sydney at Westmead Hospital, Westmead NSW 2145, Australia), "CYP2E1 and CYP4A as microsomal catalysts of lipid peroxides in murine nonalcoholic steatohepatitis." The Journal of Clinical Investigation, v. 10(8), 1067-1075.

Methionine/Choline Deficient Diet Composition:

IngredientAmount (g/kg diet)
Sucrose455.3 gm
Corn Starch200.0 gm
Corn Oil100.0 gm
Alphacel Non-Nutritive Bulk30.0 gm
AIN 76 Mineral Mix (composition below)35.0 gm
      (below is gm/kg of AIN 76 Mineral Mix)
      Calcium Phosphate Dibasic
      500.00 gm
      Sodium Chloride
      74.00 gm
      Potassium Citrate Monohydrate
      220.00 gm
      Potassium Sulfate
      52.00 gm
      Magnesium Oxide
      24.00 gm
      Manganese Carbonate (43-48% Mn)
      3.50 gm
      Ferric Citrate (16-17% Fe)
      6.00 gm
      Zinc Carbonate (70% ZnO)
      1.60 gm
      Cupric Carbonate (53-55% Cu)
      0.30 gm
      Potassium Iodate
      0.01 gm
      Sodium Selenite
      0.01 gm
      Chromium Potassium Sulfate
      0.55 gm
      Sucrose, finely powdered
      118.00 gm
Dicalcium Phosphate3.0 gm
L-Alanine3.5 gm
L-Arginine Hydrochloride12.1 gm
L-Asparagine Monohydrate6.0 gm
L-Aspartic Acid3.5 gm
L-Cystine3.5 gm
L-Glutamic Acid40.0 gm
Glycine23.3 gm
L-Histidine Hydrochloride4.5 gm
L-Isoleucine8.2 gm
L-Leucine11.1 gm
L-Lysine Hydrochloride18.0 gm
L-Phenylalanine7.5 gm
L-Proline3.5 gm
L-Serine3.5 gm
L-Threonine8.2 gm
L-Tryptophan1.8 gm
L-Tyrosine5.0 gm
L-Valine8.2 gm
DL-alpha-Tocopherol Acetate (250 u/gm)0.484 gm
Vitamin A Palmitate (250,000 u/gm)0.0792 gm
Vitamin D3 (400,000 u/gm)0.0055 gm
Ethoxyquin0.02 gm
Vitamin Mix
    Biotin
0.0004 gm
    D-Calcium Pantothenate
0.0661 gm
    Folic Acid
0.002 gm
    Inositol
0.1101 gm
    Menadione
0.0496 gm
    Niacin
0.0991 gm
    p-Aminobenzoic Acid
0.1101 gm
    Pyridoxine Hydrochloride
0.0220 gm
    Riboflavin
0.022 gm
    Thiamine Hydrochloride
0.022 gm
    Vitamin B12 (0.1% trit.)
0.0297 gm
    Ascorbic acid
1.0166 gm
    Corn Starch
3.4503 gm

Methionine/Choline Control Diet Composition:

This diet has the same formulation as the Methionine/Choline Deficient diet except we add 2 g/kg choline chloride and 3 g/kg DL-Methionine at the expense of sucrose.

Availability:

Catalog NumberDescriptionSizes
960438Methionine/Choline Deficient Diet, powdered10 kg
20 kg
50 kg
960439Methionine/Choline Deficient Diet, Pelleted10 kg
20 kg
50 kg
960440Methionine/Choline Control Diet, powdered10 kg
20 kg
50 kg
960441Methionine/Choline Control Diet, Pelleted10 kg
20 kg
50 kg